Dengue em três distritos sanitários de Belo Horizonte, Brasil: inquérito soroepidemiológico de base populacional, 2006 a 2007 Dengue fever in three sanitary districts in the city of Belo Horizonte, Brazil: a population-based seroepidemiological survey, 2006 to 2007

OBJETIVO: Determinar a soroprevalência para sorotipos virais da dengue em três distritos sanitários de Belo Horizonte e investigar a associação com variáveis de contexto e individuais. MÉTODOS: O inquérito foi conduzido nos distritos sanitários de Venda Nova, Leste e Centro-Oeste entre junho de 2006 e março de 2007. Foram incluídos todos os residentes com idade Ž 1 ano. Os participantes responderam a questionário; foi também coletada uma amostra de sangue (5 mL) para determinar a presença de anticorpos contra vírus da dengue dos tipos 1, 2 e 3 por soroneutralização. O questionário abrangeu questões demográficas, posição socioeconômica, características físicas do local de moradia, mobilidade de moradia entre cidades, história pregressa de sinais e sintomas associados à dengue e conhecimento sobre medidas de prevenção da dengue, dentre outros. RESULTADOS: Entre os 709 indivíduos estudados, encontrou-se soroprevalência para dengue de 11,9% (IC95%: 9,7 a 14,6), não associada ao sexo, idade, renda familiar e mudança de município nos últimos 10 anos. Houve associação da soropositividade com tipo de moradia (apartamento ou casa/barracão, sendo apartamento fator de proteção) e com índice elevado de vulnerabilidade da saúde do local de moradia. CONCLUSÃO: A soroprevalência neste estudo foi mais baixa do que a encontrada em outros inquéritos realizados em cidades brasileiras de porte grande e médio, sugerindo que Belo Horizonte tem utilizado estratégias eficazes de controle. Entretanto, foi observada uma heterogeneidade intraurbana na transmissão da dengue, em grande parte associada a indicadores contextuais de vulnerabilidade. Permanece ainda elevado o número de suscetíveis, e a dengue como questão de saúde pública de difícil controle.OBJECTIVE: To determine the seroprevalence of dengue fever serotypes in three sanitary districts in the city of Belo Horizonte and investigate the association of seroprevalence with contextual and individual variables. METHOD: The survey was conducted in the sanitary districts of Venda Nova, Leste and Centro-Oeste between June 2006 and March 2007. All residents aged 1 year or older were eligible for the study. Participants answered a questionnaire and had a 5 mL blood sample collected to determine the presence of anti-dengue types 1, 2, and 3 virus antibodies by seroneutralization. The questionnaire covered demographic aspects, socioeconomic status, physical characteristics of the home, residential mobility between cities, previous history of signs and symptoms associated with dengue fever, and knowledge concerning dengue fever prevention measures, among others. RESULTS: Seroprevalence was 11.9% (95%CI: 9.7-14.6) among the 709 individuals included in the study, and it was not associated with sex, age, family income, and having moved to another town in the past 10 years. Seropositivity was associated with type of construction (apartment or house/shanty, with apartment being a protection factor) and with an elevated health vulnerability index where the dwelling was located. CONCLUSIONS: In this study, seroprevalence was lower than in previous studies carried out in mid-size and large Brazilian cities. This suggests that Belo Horizonte has employed efficient control measures. However, heterogeneity within the city was observed in terms of dengue fever transmission, which was largely associated with contextual indicators of vulnerability. The number of susceptibles is still high, and the control of dengue fever remains a difficult public health issue

Mycobacteria mobility shift assay: a method for the rapid identification of Mycobacterium tuberculosis and nontuberculous mycobacteria

Submitted by Nuzia Santos ([email protected]) on 2015-02-27T12:50:33Z No. of bitstreams: 1 2014_112.pdf: 385803 bytes, checksum: 73ae8e5baf37fce92c3d713b7f570aed (MD5)Approved for entry into archive by Nuzia Santos ([email protected]) on 2015-02-27T12:50:39Z (GMT) No. of bitstreams: 1 2014_112.pdf: 385803 bytes, checksum: 73ae8e5baf37fce92c3d713b7f570aed (MD5)Approved for entry into archive by Nuzia Santos ([email protected]) on 2015-02-27T12:56:22Z (GMT) No. of bitstreams: 1 2014_112.pdf: 385803 bytes, checksum: 73ae8e5baf37fce92c3d713b7f570aed (MD5)Made available in DSpace on 2015-02-27T12:56:22Z (GMT). No. of bitstreams: 1 2014_112.pdf: 385803 bytes, checksum: 73ae8e5baf37fce92c3d713b7f570aed (MD5) Previous issue date: 2014CNPq, LMW and MLBUniversidade Federal de Santa Catarina. Laboratório de Biologia Molecular e Micobactérias. Florianópolis, SC, BrasilUniversidade Federal de Santa Catarina. Laboratório de Biologia Molecular e Micobactérias. Florianópolis, SC, BrasilUniversidade Federal de Santa Catarina. Laboratório de Biologia Molecular e Micobactérias. Florianópolis, SC, BrasilUniversidade Federal de Santa Catarina. Laboratório de Biologia Molecular e Micobactérias. Florianópolis, SC, BrasilUniversidade Federal de Santa Catarina. Laboratório de Biologia Molecular e Micobactérias. Florianópolis, SC, BrasilUniversidade Federal de Santa Catarina. Laboratório de Biologia Molecular e Micobactérias. Florianópolis, SC, BrasilUniversidade Federal de Santa Catarina. Laboratório de Protozoologia. Florianópolis, SC, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, BrasilLaboratório Central do Estado de Santa Catarina. Florianópolis, SC, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Laboratório de Vírus. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Laboratório de Vírus. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Laboratório de Vírus. Belo Horizonte, MG, BrasilThe identification of mycobacteria is essential because tuberculosis (TB) and mycobacteriosis are clinically indistinguishable and require different therapeutic regimens. The traditional phenotypic method is time consuming and may last up to 60 days. Indeed, rapid, affordable, specific and easy-to-perform identification methods are needed. We have previously described a polymerase chain reaction-based method called a mycobacteria mobility shift assay (MMSA) that was designed for Mycobacterium tuberculosis complex (MTC) and nontuberculous mycobacteria (NTM) species identification. The aim of this study was to assess the MMSA for the identification of MTC and NTM clinical isolates and to compare its performance with that of the PRA-hsp65 method. A total of 204 clinical isolates (102 NTM and 102 MTC) were identified by the MMSA and PRA-hsp65. For isolates for which these methods gave discordant results, definitive species identification was obtained by sequencing fragments of the 16S rRNA and hsp65 genes. Both methods correctly identified all MTC isolates. Among the NTM isolates, the MMSA alone assigned 94 (92.2%) to a complex or species, whereas the PRA-hsp65 method assigned 100% to a species. A 91.5% agreement was observed for the 94 NTM isolates identified by both methods. The MMSA provided correct identification for 96.8% of the NTM isolates compared with 94.7% for PRA-hsp65. The MMSA is a suitable auxiliary method for routine use for the rapid identification of mycobacteri