Characterization of novel components of the baculovirus per os infectivity factor complex

Baculovirus occlusion-derived virus (ODV) infects insect midgut cells under alkaline conditions, a process mediated by highly conserved per os infectivity factors (PIFs), P74 (PIF0), PIF1, PIF2, PIF3, PIF4, and PIF5 (ODV-E56). Previously, a multimolecular complex composed of PIF1, PIF2, PIF3, and P74 was identified which was proposed to play an essential role during ODV entry. Recently, more proteins have been identified that play important roles in ODV oral infectivity, including PIF4, PIF5, and SF58, which might work in concert with previously known PIFs to facilitate ODV infection. In order to understand the ODV entry mechanism, the identification of all components of the PIF complex is crucial. Hence, the aim of this study was to identify additional components of the PIF complex. Coimmunoprecipitation (CoIP) combined with proteomic analysis was used to identify the components of the Autographa californica multiple nucleopolyhedrovirus (AcMNPV) PIF complex. PIF4 and P95 (AC83) were identified as components of the PIF complex while PIF5 was not, and this was confirmed with blue native PAGE and a second CoIP. Deletion of the pif4 gene impaired complex formation, but deletion of pif5 did not. Differentially denaturing SDS-PAGE further revealed that PIF4 forms a stable complex with PIF1, PIF2, and PIF3. P95 and P74 are more loosely associated with this complex. Three other proteins, AC5, AC68, and AC108 (homologue of SF58), were also found by the proteomic analysis to be associated with the PIF complex. Finally the functional significance of the PIF protein interactions is discussed

Design Practice and ‘Designing for All’

Accepted for publicationIt is essential that all designers with responsibility for the human-machine interface have access to information on the anthropometry and capabilities of the whole population of people who may wish to interact with the design in question. Current databases used by designers typically present only very limited information concerning people who are older and/or disabled. Furthermore, tables of data are known to be largely ineffective and designers prefer to see visualisations of design data. In order to establish the current situation regarding design in relation to the needs of older and disabled people, existing products, procedures and systems were investigated. The objective was to identify current practice and the needs of designers whilst attempting to ‘design for all’. This paper will report on the findings from these interviews to date, which will ultimately lead to a requirements specification to aid design for the needs of older and disabled people

Being a ‘good’ mother: Immigrant mothers of disabled children

In South Korea, disability and immigration are hidden and invisible phenomena. This article examines how immigrant mothers experience the intersection of immigrant status and mothering a disabled child. Through semi-structured interviews with 16 immigrant mothers, this study found that the values of Confucian familism regarding motherhood and gender roles influenced how these immigrant mothers behaved. The study also found that immigrant mothers faced multiple forms of stigma and discrimination as a result of negative socio-cultural views of disability and immigration. Therefore, the article suggests a need for inter-professional, anti-oppression work strategies to support immigrant mothers of disabled children, along with education for social workers about these issues