Effect of bifidobacteria on intestinal injury and flora in a mouse model of ulcerative colitis

Purpose: To investigate the effect of bifidobacteria on intestinal injury and flora in a mouse model of ulcerative colitis (UC).Methods: Mouse model of UC was produced using dextran sulphate sodium (DSS). The mice were divided into seven groups, viz, reference group, MRS-L medium-negative control group, mesalaminepositive control group, high dose bifidobacteria (MIMBb75) group, middle dose MIMBb75 group and low dose MIMBb75 group. Normal mice were used as control. All mice were sacrificed at day 7 of treatment, and colon length, hemoglobin concentration and intestinal flora were determined.Results: Bifidobacteria inhibited UC-induced decreases in mice hemoglobin and UC-induced colon shortening. In addition, it augmented the diversity of intestinal flora and increased the number of bacteroides and Clostridium leptum.Conclusion: Bifidobacteria plays a therapeutic role in UC via regulation of intestinal microflora.Keywords: Bifidobacteria, MIMBb75, Ulcerative colitis, Intestinal injury, Intestinal flor

Case Report: A rare synchronous multiple gastric carcinoma achieved progression-free disease through NGS-guided serial treatment

Synchronous multiple gastric carcinoma (SMGC) is a rare condition characterized by the simultaneous occurrence of two or more primary malignant tumors in the stomach, each with its own distinct pathological morphology. SMGC differs from gastric metastases, which originate from primary gastric or non-gastric tumors. At present, the incidence of SMGC is low in China, with no established guidelines for standard treatment. Here, we report a rare case of advanced SMGC that achieved long-lasting clinical benefits through a treatment strategy informed by next-generation sequencing (NGS). Dynamically monitoring of the tumor and/or circulating cell-free DNA guided the patient’s treatment sequentially. The patient received anti-HER2 therapy, followed by immunotherapy, pembrolizumab in combination with trastuzumab and chemotherapy, and ultimately underwent successful total gastrectomy. This case highlights a novel approach of utilizing liquid biopsy-based NGS to gain insights into disease progression and molecular response to NGS-guided treatment in SMGC patients

Retracted “Effect of bifidobacteria on intestinal injury and flora in a mouse model of ulcerative colitis”

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