ソバ種実から得られる低分子性タンパク質の精製と2,3の理化学的性質

Two low molecular weight proteins were separately isolated in crystalline forms from buckwheat seeds by affinity chromatography on a chitin column, ion exchange chromatography on a CM-cellulose column and gel filtration through a Sephadex G-25 column. The two crystalline proteins thus obtained were referred to as buckwheat chitin-binding proteins I and II, BCP I and II, respectively. Further purification of the crystalline BCP II by high performance liquid chromatography resulted in separation into the three proteins, BCP IIa1, IIa2 and IIb, respectively. These three purified proteins showed a single band on SDS-polyacrylamide gel electrophoresis, indicating that each protein was essentially homogeneous. The purified BCP IIa1, IIa2 and IIb had the very similar amino acid compositions, mutually, and comprised the total numbers of amino acid residues of 40 to 46, suggesting that they are closely related to each other. The amino acid compositions of the purified proteins revealed that the minimum molecular weights of BCP IIa1, IIa2 and IIb were 4,800, 5,500 and 4,800, respectively. The proteins were stable in the wide range of pH3 to 11 at lower temperature than 60℃ and even in the range of acidic pH at 100℃

Clinical Study A Randomized Prospective Study of Bowel Preparation for Colonoscopy with Low-Dose Sodium Phosphate Tablets versus Polyethylene Glycol Electrolyte Solution

Optimal bowel preparation is essential for the safety and outcome of colonoscopy. A solution containing polyethylene glycol (PEG) is often used as a bowel cleansing agent, but some patients are intolerant of PEG, and this may lead to discontinuation of colonoscopy. Sodium phosphates (NaP) tablets are designed to improve patient acceptance and compliance. The objective of this study was to compare bowel preparation efficiency and patient acceptance of a 30 NaP tablet preparation (L-NaP) and a 2 L PEG preparation. Patients were randomized into either the L-NaP or PEG group. The primary endpoint was the efficiency of colon cleansing as assessed by a validated four-point scale according to the Aronchick scale by endoscopists and was verified by blinded investigators. The secondary endpoints were patients' tolerability and acceptance. Colon-cleansing efficiency was not significantly different between the two preparations. However, patients' overall judgment was significantly in favor of L-NaP, reflecting better acceptance of L-NaP than PEG. Additionally, more patients favored L-NaP over PEG in a hypothetical future occasion requiring colonoscopy

ソバ種実から得られる低分子性タンパク質のキチン結合活性ならびに抗真菌活性

The low molecular weight proteins which had affinity for chitin were purified in two types of crystalline forms from buckwheat seeds and named as buckwheat chitin-binding proteins I and II, BCP I and II. respectively. To elucidate biological properties of BCP II of them, chitin-binding and antifungal activities of the protein were examined in detail. BCP II possessed the binding specificity for β-1, 4-linked N-acetylglucosamine polysaccharide, chitin, and bound to chitin, reversibly. It was found that the incubation of the protein with chitin at 30℃ and pH 8.0 for 20min was most suitable for the chitin-binding. The equilibrium absorption constant, Ka, and maximum amount of the bound protein, [PC]max, values for binding of BCP II with chitin were estimated to be 0.03 1/μmol and 18.2/μmol/g, respectively. This [PC]max value indicates that approximately 87.3mg of the protein utilizes 1g of chitin for binding. BCP II also showed the growth inhibition against fungi, Saccharomyces, Candida, Aspergillus and Penicillum tested, though the growth of bacteria was not inhibited by the protein, suggesting that the protein may play a important role as defense protein against invasion of plant pathogenic fungi into seeds

Impact of relative dose intensity (RDI) in CHOP combined with rituximab (R-CHOP) on survival in diffuse large B-cell lymphoma

<p>Abstract</p> <p>Background</p> <p>Recently, maintaining higher relative dose intensity (RDI) of chemotherapeutic drugs has become a widespread practice in an attempt to achieve better outcomes in the treatment of aggressive lymphoma. The addition of rituximab to chemotherapy regimens has significantly improved outcome in diffuse large B-cell lymphoma (DLBL). However, it is unknown if higher RDI in chemotherapy when combined with rituximab leads to a better outcome in aggressive B-cell lymphoma.</p> <p>Methods</p> <p>We retrospectively evaluated the impact of the RDI of initial chemotherapy (consisting of cyclophosphamide, doxorubicin, vincristine and prednisolone with rituximab (R-CHOP) on outcome in 100 newly diagnosed DLBL patients.</p> <p>Results</p> <p>A multivariate Cox regression model showed that RDI trended towards a significant association with mortality [hazard ratio per 0.1 of RDI = 0.8; 95% confidence interval 0.6–1.0; <it>P </it>= 0.08]. Additionally, on multivariate logistic analysis, advanced age was a significant factor for reduced RDI.</p> <p>Conclusion</p> <p>Our data suggest that in DLBL patients, mortality was affected by RDI of R-CHOP as the initial treatment, and the retention of a high RDI could therefore be crucial.</p

子育て世代対象の健康教室を通した地域コミュニティの活性化

我々保健師学生は、地域コミュニティーを活性化させる取り組みとして、小学生の子どもをもつ成人期の住民を対象に生活習慣病予防について親子健康教室を実施した。健康教室実施のために地域の関係者と行った協働活動や教室後の調査結果から、地域コミュニティーの活性化に必要な支援方法として以下の5つが明らかになった。1.顔の見える関係づくり・関係者と協働した呼び掛け、2.関係者同士をつなぎ、意欲を引き出す、3.地域のニーズを把握し、住民の視点を取り入れた活動内容にする、4.成人期の参加を促すためには親子を対象にする、5.親子療法へのアプローチ

Inferring a System of Differential Equations for a Gene Regulatory Network by using Genetic Programming

This paper describes an evolutionary method for identifying the gene regulatory network from the observed time series data of the gene&apos;s expression. We use a system of ordinary differential equations as a model of the network and infer their right-hand sides by using Genetic Programming (GP). To explore the search space more effectively in the course of evolution, the least mean square (LMS) method is used along with the ordinary GP. We apply our method to three target networks and empirically show how successfully GP infers the systems of differential equations