Usage Tests of Oak Moss Absolutes Containing High and Low Levels of Atranol and Chloroatranol.

Atranol and chloroatranol are strong contact allergens in oak moss absolute, a lichen extract used in perfumery. Fifteen subjects with contact allergy to oak moss absolute underwent a repeated open application test (ROAT) using solutions of an untreated oak moss absolute (sample A) and an oak moss absolute with reduced content of atranol and chloroatranol (sample B). All subjects were in addition patch-tested with serial dilutions of samples A and B. Statistically significantly more subjects reacted to sample A than to sample B in the patch tests. No corresponding difference was observed in the ROAT, though there was a significant difference in the time required to elicit a positive reaction. Still, the ROAT indicates that the use of a cosmetic product containing oak moss absolute with reduced levels of atranol and chloroatranol is capable of eliciting an allergic reaction in previously sensitised individuals

Patch Testing with a Textile Dye Mix in a Baseline Series in Two Countries

Disperse dyes are the most common contact sensitizers among textile dyes. The main aim of this study was to investigate the outcome of patch testing with a textile dye mix 6.6%. A total of 2,049 patients from Sweden and 497 from Belgium were tested with the mix, consisting of Disperse (D) Blue 35, D Yellow 3, D Orange 1 and 3, D Red 1 and 17, 1.0% each, and D Blue 106 and D Blue 124, 0.3% each. Of the total number, 65 patients, 2.6%, tested positively to the mix, 4.2% of the Belgian patients and 2.1% of the Swedish patients. Patch testing with the mix 6.6% revealed significantly more patients with contact allergy compared with testing with a previous mix 3.2% (p<0.01). Contact allergy to the mix was significantly more common in the Belgian than in the Swedish patients

In VivoEvaluation of the Protective Capacity of Different Gloves Against Hair Dyes

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Contact allergens in p-tert-butylphenol-formaldehyde resin

p-tert-Butylphenol-formaldehyde resin (PTBP-F-R) is used as a binder in many adhesive formulations and it consists of a complex mixture of substances, most of which are unknown. The resin has been reported to cause allergic contact dermatitis since the 1950s. The aim of the study was to investigate allergens in p-tert-butylphenol-formaldehyde resin (PTBP-F-R) and to isolate and identify sensitizers that are important factors for the development of hypersensitivity to PTBP-F-R in man. Chemical separation methods were used to isolate fractions and pure substances from the resin. These were patch tested in patients hypersensitive to PTBP-F-R. The sensitizing capacities and cross-reaction patterns of 11 isolated substances were investigated using the Guinea pig maximization test. In the present investigation 7 new allergens in PTBP-F-R were established and 1 more was strongly indicated. The allergens were found among monomers, dimers and trimers. Patch testing of formaldehyde and 4-tert-butyl-phenol, the raw materials for production of PTBP-F-R, indicated that these substances are not frequent allergens among patients hypersensitive to PTBP-F-R. 5-tert-Butyl-2-hydroxy-3-hydroxymethyl-benzaldehyde was considered to be an important allergen among the monomers. The dimers 4-tert-butyl-2-(5-tert-butyl-2-hydroxy-3-hydroxymethyl-benzyloxymethyl)-6-hydroxymethyl-phenol and 4-tert-butyl-2-(5-tert-butyl-2-hydroxy-benzyloxymethyl)-6-hydroxymethyl-phenol were considered main allergens in the resin. The investigated substances were quantitatively determined in 2 PTBP-F-Rs and shown to be present in concentrations varying between 0.01 and 1.7% w/w

Risks and possibilities in patch testing with contaminated personal objects: usefulness of thin-layer chromatograms in a patient with acrylate contact allergy from a chemical burn

We report a case of a chemical burn from dipropylene glycol diacrylate (DPGDA) spilt on working shoes, followed by active sensitization, thus giving an occupational allergic contact dermatitis on the patient's dorsal feet. Diagnostic tests included patch testing with acetone extracts made from the different shoe layers and thin-layer chromatograms. An invisible spot on the thin-layer chromatography plate gave a test eczema and was further investigated with gas chromatography-mass spectrometry. DPGDA was detected in the spot