Samples.

<p>Samples.</p

Sequence analysis identifies a variety of viruses in samples from febrile and afebrile children.

<p>Analysis of 176 plasma and NP samples on the Illumina GAIIX and HiSeq 2000 platforms identified approximately 50,000 sequences with similarity to 25 known (A) DNA and (B) RNA virus genera.</p

Virus genera screened by PCR.

<p>Virus genera screened by PCR.</p

Viruses detected by sequencing that were not screened by PCR.

<p>The bars represent the number of samples in which each virus was detected by sequencing. *Indicates viruses that were not assayed by PCR in NP samples. **Indicates a virus that was not assayed by PCR but belongs to a genus with members that were.</p

Febrile children have more viral sequences from a greater range of viruses than do afebrile children.

<p>The number of sequences was scaled to 3 million per sample before comparisons were made between groups. (A) The average numbers of viral sequences found in plasma and NP samples from the subjects are represented by gray bars for samples from afebrile children and black bars for samples from febrile children. The percentage of samples in each group for which 0, 1, 2, 3, 4, or 5 viruses was detected is plotted for (B) sequencing data and (C) PCR data.</p

Detection of viruses with next generation sequencing.

<p>Detection of viruses with next generation sequencing.</p

Illumina sequences with remote homologies to astroviruses.

<p>The top alignment from tblastx, excluding MLB1 and MLB2, is reported if the alignment was to an astrovirus.</p

Genome coverage.

*<p>Full genome sequence not available. Largest Genbank sequence used.</p

Comparison of sequencing and PCR results.

<p>The number of samples in which each virus was detected by PCR (white bars), sequencing (gray bars), or both (black bars) is shown.</p