Gene expression profile comparisons between Fenholloway (PME) and reference site (Econ) female <i>G. holbrooki</i> and female <i>G. holbrooki</i> exposed to the androgen 17β-trenbolone (TB) or the vehicle control (C).

<p>This set of genes were differentially expressed between the PME, Econfina, and TB groups over the lab controls with at least a 1.5-fold difference of expression over the lab control and was expressed in the same direction in those groups. Data were median-centered by gene and clustered using spearman correlation and centroid linkage. Yellow genes are more highly expressed than the gene average and blue genes are expressed at a lower level than the gene average.</p

Focused hepatic gene expression patterns in female Eastern mosquitofish (<i>Gambusia holbrooki</i>) using qualitative polymerase chain reaction analysis.

<p>Gene expression of (A) vitellogenin (<i>vtg</i>), (B) zona pellucida glycoprotein 2 (<i>zp2</i>), (C) 17β-hydroxysteroid dehydrogenase 3 (<i>17βhsd3</i>) was analyzed between paper mill exposed (Fenholloway) and reference (Econfina) collection sites. Each point represents the mean of each group and error bars represent standard deviation; time points with no standard deviation have an N of 1. A box indicates statistical significance between groups at the selected oocyte developmental stages as determined using a Student's t-test with p<0.05.</p

Significantly differentially regulated biological processes in female <i>G. holbrooki</i> residing downstream of a paper mill as compared to a control site as determined by gene set enrichment and gene ontology (GO) analyses.

a<p> Percentage of genes within the GO category that were significantly differentially regulated between Econfina and Fenholloway groups.</p>b<p> Percentage of genes within the GO category that were not significantly differentially regulated between Econfina and Fenholloway groups.</p><p>Significantly differentially regulated biological processes in female <i>G. holbrooki</i> residing downstream of a paper mill as compared to a control site as determined by gene set enrichment and gene ontology (GO) analyses.</p

Anal fin elongation and bone segment formation in anal fin ray 3 of female Eastern mosquitofish (<i>Gambusia holbrooki</i>) collected from the Fenholloway and Econfina Rivers.

<p>Data are repsresented as (A) Mean (± standard deviation) of all collection events and, (B) Distribution of anal fin elongation classes between both paper mill exposed (Fenholloway) and reference (Econfina) field sites. An asterisk indicates statistical significance between the two groups as determined using a Student's t-test with p<0.05. For anal fin elongation levels there was an N of 46 and 50 from the Fenholloway and Econfina rivers respectively and a subset N of 4 from both sites for the bone segment evaluation.</p

Results of progesterone receptor (PR) and androgen receptor (AR) GeneBLAzer assays for concentrated water samples collected downstream of the Fenholloway River paper mill and the Econfina River conservation area.

<p>The graph bars represent the mean of three replicates from each dilution for the PR (A) and AR (B) assays. Error bars represent standard deviation of the three replicates per assay. The dose-responses of levonorgestrel, progesterone, and 17β-trenbolone (C) were also evaluated by the AR assay.</p

Anal fin gene expression patterns of sonic hedgehog (<i>shh</i>) in female Eastern mosquitofish (<i>G. holbrooki</i>).

<p>An asterisk indicates statistical significance between the paper mill exposed (Fenholloway) and reference (Econfina) rivers as determined using a Student's t-test with p<0.05.</p