Clinical characteristics of the study population.

<p>Clinical characteristics of the study population.</p

Protein quantified in the targeted proteomics analysis.

<p>Protein quantified in the targeted proteomics analysis.</p

Protective effects of HDL on endothelial cells are impaired in diabetes, correlate with S1P and negatively associate with <i>in vivo</i> measure of endothelial dysfunction.

<p><b>(A)</b> The ability of HDL to suppress NFκB activation was measured as phosphorylation of p65 in HMEC after 16 h incubation with HDL (50 μg/mL) followed by 4 h stimulation with TNFα (n = 41 per group). <b>(B)</b> The ability of HDL to stimulate eNOS Ser1179 phosphorylation was measured in BAEC after 30 min incubation with HDL (50 μg/mL) (n = 38 non-diabetic, n = 41 diabetic subjects). (Data is expressed relative to cells not treated with HDL). <b>(C)</b> Sphigosine-1-phosphate concentration measured by LC-MS is reduced in patients with diabetes and <b>(D)</b> correlates positively with HDL ability to stimulate eNOS phosphorylation (n = 40 non-diabetic, n = 39 diabetic subjects; *excluded outlier and <i>P</i>-value after exclusion). <b>(E)</b> The ability of HDL to stimulate eNOS is inversely correlated negatively with level of P-selectin in plasma, an <i>in vivo</i> measure of endothelial dysfunction (Pearson correlation coefficient; n = 81 after an outlier exclusion).</p

Odds ratios for type 2 diabetes according to HDL endothelial protective functions and selected dyslipidemia and common risk factors.

<p>Multivariate logistic regression models included the listed dyslipidemia and other cardiovascular risk factors and odds ratios for continuous variables are presented per 1-SD increase for <b>(A)</b> HDL ability to stimulate eNOS activation, and <b>(B)</b> for the suppression of NFκB activation.</p

Vaisar et al, Type 2 Diabetes is Associated with Loss of HDL Endothelium Protective Functions

Targeted proteomics analysis of HDL from people with and without T2D. Attached files contain Skyline documents with relevant SRM-LCMS data. Data is directly accessible at PanoramaWeb Public, https://panoramaweb.org/labkey/VaisarHDL_T2D.url.<br>Original publication - Vaisar et al, PlosOne 2018.<br