1 research outputs found
Using Synthetic Biological Parts and Microbioreactors to Explore the Protein Expression Characteristics of <i>Escherichia coli</i>
Synthetic
biology has developed numerous parts for the precise
control of protein expression. However, relatively little is known
about the burden these place on a host, or their reliability under
varying environmental conditions. To address this, we made use of
synthetic transcriptional and translational elements to create a combinatorial
library of constructs that modulated expression strength of a green
fluorescent protein. Combining this library with a microbioreactor
platform, we were able to perform a detailed large-scale assessment
of transient expression and growth characteristics of two <i>Escherichia coli</i> strains across several temperatures. This
revealed significant differences in the robustness of both strains
to differing types of protein expression, and a complex response of
transcriptional and translational elements to differing temperatures.
This study supports the development of reliable synthetic biological
systems capable of working across different hosts and environmental
contexts. Plasmids developed during this work have been made publicly
available to act as a reference set for future research