Growth of MIA PaCa-2 tumors xenografted into nude mice.

<p>a) Tumor volumes monitored by bioluminescence for the 48 days of the experiment. Values represent means ±SEM, <i>n</i> = 9 for each group. b) Exemplary results from <i>in vivo</i> BLI, showing one non treated mouse with loss of BLI signal.</p

Tumor volume after orthotopic injection of transduced or non transduced MIA PaCa2 cells.

<p>Tumor volumes were measured with a caliper at the day of euthanasia (D36). The columns represent the means (±SEM), <i>n</i> = 7 for each group.</p

Immunohistochemistry of pancreatic tumor sections 48 days post MIA PaCa2-luc cell injection (Bar = 100

<p> <b>µm).</b> a) Anti-luciferase antibody labeled with TRITC (red) confirmed luciferase expression in tumor sections; b and c) <i>In situ</i> detection of hypoxic tumor regions by using anti-pimonidazole antibody labeled with FITC (green). b) An intensive hypoxic tumor regions can be seen at the center. Positive immunosignals were also seen at the periphery c).</p

a) Schematics of the plasma gun setup; b) Therapeutic schedule of gemcitabine and plasma gun.

<p>a) Schematics of the plasma gun setup; b) Therapeutic schedule of gemcitabine and plasma gun.</p

Impact of the transduction on MIA PaCa2 cells; a) <i>In vitro</i> cell growth of transduced and non transduced MIA PaCa2 cells.

<p>Each point represents the mean (±SEM), <i>n</i> = 3 for each group. b) Cells response to gemcitabine alone <i>in vitro</i>. Cell viability was determined 24 h after treatment by MTT and was normalized to untreated cells. Each point represents the mean (±SEM), <i>n</i> = 5 for each group.</p

Inhibitory effect of NTP on cell proliferation <i>in vitro</i> in MIA PaCa2-luc cells.

<p>Cells were treated with NTP alone or in combination with gemcitabine. Cell viability was determined 24 h after treatment by bioluminescence and was normalized to untreated cells. Each point represents the mean (±SEM), <i>n</i> = 5 for each group.</p

ATR-FTIR spectra of the PE film for untreated and 120 min plasma treatment at 100 W of O₂, Ar and N₂.

<p>ATR-FTIR spectra of the PE film for untreated and 120 min plasma treatment at 100 W of O₂, Ar and N₂.</p

Scanning electron micrograph of <i>P</i>. <i>aeruginosa</i> before and after plasma treatments (25 W; 14 G and 0.5 sccm), (G x 7,500) (Jeol JSM 5400LV).

<p>(A) Untreated. (B, E) After N₂ plasma treatment. (C, F) After Ar plasma treatment. (D, G) After O₂ plasma treatment.</p

Scanning electron micrograph of <i>B</i>. <i>subtilis</i> spores before and after O₂ gas plasma treatments (100 W; 14 G and 1 sccm), (G x 7,500 for the left column and G x 20,0000 for the right column).

<p>(A-B) Untreated. (C-F) After O₂ plasma treatment.</p

Plasma sterilization prototype device and creation of plasma inside sealed bags.

<p>(A) The vacuum chamber was equipped with 2 pumps, a radio frequency polarization plate and two magnetic coils. (B) Creation of N₂- gas plasma inside a sealed bags.</p