IFN-γ suppresses the accumulation of neutrophils.

<p>Animals were inoculated i.p. with 500 μg/mouse normal rat IgG (A) or anti-IFN-γ (B) at 1 hour prior to surgery. The animals were euthanized on day 3 post-surgery, the NPCs were isolated, and the neutrophils were stained (Ly-6G/CD11b) and quantified by flow cytometry. A summary of experimental results is presented in the table. Data are derived from a single experiment representative of two independent experiments, n  =  3-6 mice/group.</p

Kupffer cells induce the activation and accumulation of iNKT cell in biliary obstructed livers.

<p>Animals were treated with Cl₂MDP-L to deplete Kupffer cells prior to BDL; control mice received PBS. At 18 hours post-BDL, the NPCs were teased through screens, counted, purified on Percoll gradients and stained. Hepatic, CD1d-tetramer⁺ iNKT cells were quantified (A), and the expression of CD25 (B), CD69 (C), and ICAM-1 (D) was determined by flow cytometry. Values in parentheses denote percentages of expression. A summary of experimental results is presented in table format containing cell numbers and statistical analyses. Data are derived from three independent experiments, n  =  3-6 mice/group. n/a  =  not available; small sample size precludes statistical analysis.</p

LFA-1-dependent activation and accumulation of iNKT cells in cholestatic livers.

<p>BDL WT mice were treated with anti-LFA-1 monoclonal antibody or normal rat IgG at 1 hour prior to BDL. Animals were euthanized at 18 hours post-surgery, the NPC were purified on a Percoll gradient, and the iNKT cell markers were stained. CD1d-tetramer⁺ iNKT cells were quantified (A) and CD25 expression was determined (B) by flow cytometry. Values in parentheses denote percentages of expression. A summary of experimental results is presented in table format containing cell numbers and statistical analyses. Data are derived from three independent experiments, n = 3-6 mice/group.</p

Liver injury is significantly increased in the absence of iNKT cells.

<p>Plasma ALT (A) and AST (B) levels were determined in iNKT cell-deficient and WT mice at three days post-BDL. Representative liver sections were collected from the WT (C) and iNKT^−/− (D) mice, embedded in paraffin, sectioned, stained with Hematoxylin & Eosin, and scored. The livers of BDL iNKT^−/− mice exhibited significantly greater areas of necrosis (indicated by black arrows) (E). Data are derived from four independent experiments, n  =  3-5 mice/group. *Significantly greater than sham-operated controls, <i>P</i> <0.05; **Significantly greater than BDL, WT mice, <i>P</i> <0.05 (Student’s <i>t</i>-test).</p