An <i>in vitro</i> Autogene

Recent technological advances have allowed development of increasingly complex systems for <i>in vitro</i> evolution. Here, we describe an <i>in vitro</i> autogene composed of a self-amplifying T7 RNA polymerase system. Functional autogene templates in cell-free lysate produce T7 RNA polymerase, which amplifies the autogene genetic information through a positive feedback architecture. Compartmentalization of individual templates within a water-in-oil emulsion links genotype and phenotype, allowing evolution

Appendix A. Univariate statistical procedure and detailed responses of vegetation structure.

Univariate statistical procedure and detailed responses of vegetation structure

Appendix B. Quantification and detailed responses of tree species diversity.

Quantification and detailed responses of tree species diversity

Appendix C. Detailed responses of absolute tree species woody biomass.

Detailed responses of absolute tree species woody biomass