Evaluation of Light-Emitting Diode (LED-660 Nm) Application over Primary Osteoblast-Like Cells on Titanium Surfaces: An In Vitro Study

Background: The goal of this study was to evaluate the behavior of neonatal rat calvarial osteoblast-like cells cultured on different implant surfaces and exposed once or three times to a 660-nm light-emitting diode (LED)

Protective Effects of Berberis Vulgaris Plant Extract on Paracetamol-induced Liver Toxicity in Rats

INTRODUCTION: Berberis vulgaris extract is known to be protective against many damages due to its anti-inflamatory and antioxidant properties. Paracetamol toxicity is one of the most common toxicities. In our study, we aimed to reduce the damage caused by paracetamol toxicity with the beneficial effects of Berberis vullgaris extract. METHODS: In our study, 42 Sprague Dawley 8-week-old rats were used. 7 groups were formed with 6 female animals in each group. At the 24th hour of the study, all groups underwent laparotomy under anesthesia and liver dissection was performed. Hematoxylin and Eosin (H&E) staining was used to evaluate liver histopathology, and PAS staining was used to show glycogen and basement membrane staining in liver tissue. In addition, scoring was done by showing apoptotic cells with TUNEL stain RESULTS: Hepatic degeneration and peritubular inflammation were quite evident in the groups to which we applied paracetamol. In this study, we observed that the number of positive cells increased in the group given paracetamol in TUNEL staining. As a result of the PAS staining we performed, glycogen accumulation in the hepatocytes of the group we induced with paracetamol was remarkable. DISCUSSION AND CONCLUSION: Berberis vulgaris plant extract appears to reduce Paracetamol-induced hepatic toxicity. As a result of the PAS staining, glycogen accumulation in the hepatocytes was remarkable in the group which was induced with paracetamol. Further studies are required for the use of determined molecules of Berberis vulgaris plant extract against the hepatotoxic effect of paracetamol in paracetamol toxicity

The prevelance of mesiodens in a group of non-syndromic Turkish children: a radiographic study

Purpose The aim of the present study is to determine the prevelance and clinical status of mesiodens in a group of non-syndromic Turkish children, with an analysis of the associated clinical-eruptive complications. Materials and Methods This study sample consists of 58142 pediatric patients’. Standard equipment and films were used in the suspected patients. The examination of all radiographs was performed under standard conditions by two pediatric dentists with over 10 years experience. Age, gender, number of mesiodens, morphology and clinical status were recorded in forms. Results A total of 83 mesiodentes were diagnosed in 59 children with ages ranging from 6-14 years. The prevelance of mesiodens was estimated as 0.1%. Males were more frequently affected than females in the ratio of 2.3:1. Of the 83 mesiodentes, 48.2% were conical, 31.3% were tuberculate and 20.5% were incisor like, 22.9% were inverted, and 68.7% were fully impacted. The number of mesiodens was one in 36 cases (61.0%), two in 22 cases (37.3%) and three in one case (1.7%). The mean age at the time of diagnosis of the mesiodens was 9.5 years. The main complication as­sociated with the mesiodens was displacement or rotation of the permanent teeth (73.3%). Conclusion This study presents 0.1% prevelance of mesiodens in a group of Turkish children sample. The majority of the mesiodentes were unilateral located in the premaxillary region, were conical shaped, and remained unerupted. The mean age at the time of diagnosis of the mesiodens in this study was 9.5 years, with this period being later than the eruption time of the maxillary central incisor