Colonization, translocation and antibody response in experimental Campylobacter infection of chickens

YARDIMCI, Hakan/0000-0002-5994-5792; Akan, Mehmet/0000-0002-7342-1450WOS: 000180152200025In this study we examined factors affecting the colonization of campylobacters in chick intestines, colonization times and elicited immune responses. In chicks, infected orally by minimal infective dose (100) (MID100), colonization in the intestines was observed to reach 100% during the 3-week period following C. jejuni and C. coli inoculation. When the SDS-PAGE protein profiles of the inoculated and isolated strains were examined similarities of 98.3% among C. jejuni strains and 98.5% among C. coli strains were determined. The results obtained from the ELISA tests performed with blood sera in the group infected with C. jejuni and in the control group for the 2nd, 3rd and 4th weeks were 0.27-0.25, 0.22-0.18 and 0.27-0.17, and the group infected with C. coli and the control group were 0.28-0.25, 0.24-0.19 and 0.32-0.18. After the immunoblotting analyses, specific bands that have molecular masses of 61, 47.5, 29.8 and 25.6 kDa were identified from C. jejuni strains and 62.5, 48 ve 31 kDa from C. coli strains, respectively

The evaluation of renal allo and autotransplantation results in cats

WOS: 000180152200023For the first time in Turkey, renal autotransplantation in 7 cats and renal allotransplantation in 7 cats having blood-crossmatch-compatible donors were performed. Immunosuppression was maintained by a prednisolone-cyclosporin combination in the renal allotransplantation group. All cats in the renal allotransplantation group died during the operation or between 3 and 72 h postoperatively. Two cats in the renal autotransplantation group survived approximately 2 years. Hyperacute or acute rejection findings were not encountered according to perioperative observations, laboratory findings or histopathological evaluations of renal allografts. The results of histopathological evaluations of renal allografts indicated acute tubular necrosis (ATN) findings caused by renal hypoperfusion due to hypotension. The reason for ATN was considered to be a result of hypotension that invasive methods could not control

The relationship between the stages of the sexual cycle, the pregnancy and postpartum periods and vaginal flora in Kangal Breed bitches

FINDIK, Murat/0000-0003-1408-2548WOS: 000184705900037This study was performed on females of the Kangal Breed (Turkish Anatolian Shepherd Dog) with the aim of determining the relationship between the stages of the sexual cycle and vaginal flora. The stages of the cycle in Kangal bitches (n = 16) were determined by performing vaginoscopic, vaginal cytologic and ultrasonographic checks at regular intervals and examining vaginal swab samples bacteriologically. The swab samples were inoculated onto 7% sheep blood agar and incubated for 18-24 h at 37 degreesC and total counts of the resulting colonies were evaluated. In addition, inoculations were made onto 7% sheep blood agar, PPLO agar, Columbia agar and Brucella agar with serum and incubated in a 10% CO2 environment at 37degreesC and onto Saboraud dextrose agar, and these were incubated at room temperature and the resulting colonies identified. In all stages of to cycle E. coli and also Streptococci, were isolated, except in the pregnancy period. It was determined that haemolytic streptococci grew at the estrus and early metestrus stages while alpha-haemolytic streptococci grew at other stages of the cycle. Staphylococci were not found at the pre-proestrus and estrus stages, whereas they were isolated in various percentages from samples taken at the other stages. At the stages following the end of estrus, Citrobacter, Flavobacter and Proteus spp. were isolated in different percentages. Enterobacter, Acinetobacter and Neisseria spp. were isolated at different stages of estrus, Pasteurella spp. at late estrus and Shigella spp. as well as yeast in the pre-proestrus periods. While the E coli was grown at a rate of 76.9% from the samples from breeding bitches, the coincidence rates of B-haemolytic streptococci, alpha-haemolytic streptococci as well as S. aureus were determined to be 15.4%, 15.4% and 38.5%, respectively. Pasteurella, Neisseria and S. epidermidis levels were the same; 7.7%. It was observed that, E. coli appeared at a level of 100% in all the non-pregnant bitches, and E. coli alone was isolated at a level of 75%. Similarly E. coli appeared in six of the bitches that became pregnant (66.7%) and E. coli was isolated alone from half of these samples (50%). As a result it was observed that some of the bacterial components comprising the vaginal flora were present at every stage of the sexual cycle, while some were absent at particular stages; bacteria could grow at the estrus stage in the vagina without influencing the outcome of the pregnancy