A New Remote Sensing-Based System for the Monitoring and Analysis of Growth and Gas Exchange Rates of Photosynthetic Microorganisms Under Simulated Non-Terrestrial Conditions

Oxygenic photosynthetic microorganisms are a focal point of research in the context of human space exploration. As part of the bioregenerative life-support systems, they could have a key role in the production of breathable O2, edible biomasses and in the regeneration of CO2 rich-atmospheres and wastewaters produced by astronauts. The test of the organism\u2019s response to simulated physico-chemical parameters of planetary bodies could also provide important information about their habitability potential. It is believed that the success of future planetary and space missions will require innovative technologies, developed on the base of preliminary experiments in custom-made laboratory facilities. In this context, simulation chambers will play a pivotal role by allowing the growth of the microorganisms under controlled conditions and the evaluation in real-time of their biomass productivity and impact on atmosphere composition. We here present a system capable of addressing these requirements with high replicability and low costs. The setup is composed by three main parts: 1) a Star Light Simulator, able to generate different light intensities and spectra, including those of non-solar stars; 2) an Atmosphere Simulator Chamber where cultures of photosynthetic microorganisms can be exposed to different gas compositions; 3) a reflectivity detection system to measure from remote the Normalized Difference Vegetation Indexes (NDVI). Such a setup allows us to monitor photosynthetic microorganism\u2019s growth and gas exchange performances under selected conditions of light quality and intensity, temperature, pressure, and atmospheres simulating non-terrestrial environments. All parameters are detected by remote sensing techniques, thus without interfering with the experiments and altering the environmental conditions set. We validated the setup by growing cyanobacteria liquid cultures under different light intensities of solar illumination, collecting data on their growth rate, photosynthetic activity, and gas exchange capacity. We utilized the reflectivity detection system to measure the reflection spectra of the growing cultures, obtaining their relative NDVI that was shown to correlate with optical density, chlorophyll content, and dry weight, demonstrating the potential application of this index as a proxy of growth

A tunable integrated system to simulate colder stellar radiation

In the last years, a lot of extrasolar planets have been discovered in any direction of the Galaxy. More interesting, some of them have been found in the habitable zone of their host stars. A large diversity of spectral type, from early types (A) to colder ones (M), is covered by the planetary system host stars. A lot of efforts are done in order to find habitable planets around M stars and indeed some habitable super earths were found. In this framework, a€Atmosphere in a Test Tubea€, a project started at Astronomical observatory of Padua, simulates planetary environmental condition in order to understand how and how much the behavior of photosynthetic bacteria in different planetary/star scenarios can modify the planet atmosphere. The particular case of an habitable planet orbiting a M dwarf star is under study for the time being. The irradiation of an M star, due to its lower surface temperature is very different in quality and quantity by the irradiation of a star like our Sun. We would like to describe the study of feasibility of a new kind of tunable led stellarlight simulator capable to recreate the radiation spectrum of M type stars (but with the potential to be expanded even to F, G, K star spectra types) incident on the planet. The radiation source is a multiple LED matrix cooled by means of air fan technology. In order to endow it with modularity this device will be composed by a mosaic of circuit boards arranged in a pie-chart shape, on the surface of which will be welded the LEDs. This concept is a smart way in order to replace blown out pieces instead of changing the entire platform as well as implement the device with new modules suitable to reproduce other type of stars. The device can be driven by a PC to raise or lower the intensity of both each LED and the lamp, in order to simulate as close as possible a portion of the star spectrum. The wavelength intervals overlap the limits of photosynthetic pigment absorption range (280-850 nm), while the range of the radiation source will be between 365 nm and 940 nm. The reason why we chose a higher outer limit is that M stars have the emission peak at about 1000 nm and we want to study the effects of low-light radiation on bacterial vitality. The innovative concept behind this radiative source is the use of the LED components to simulate the main stellar absorption lines and to make this a dynamic-light. Last but not least the use of LED is crucial to keep the device compact and handy. This device could help us to better understand the link between radiation and NIR-photosynthesis and could find applications in the field of photobioreactors as a test bench for the choice of the wavelength to be used in order to maximize the production rate. Other fields of application are the microscopy light sources field and the yeasts growth sector

Super Earths, M-Stars and Photosynthetic Bacteria

Within the atmosphere in a test tube project, we are performing laboratory simulations trying to replicate the environmental conditions of an Earth-like planet orbiting around the mean habitable zone of an M star in order to understand what kind of biomarkers could be revealed by different biotypes, if present, living therein. We describe the laboratory set-up and the the methods we used in order to analyze the O2 and CO2 photosynthetic balance using atypical photosynthetic organisms and preliminary results

Secondary omental torsion in children: a report of two cases and review of the literature.

The Authors describe a case of omental torsion in pediatric ag

Control software for the Multi-Channel Led starlight simulator

We built a multi-channel led starlight simulator capable to reproduce the radiation of stars of F, G, K and M spectral types in the wavelength range 365-940 nm. This range overlaps the photosynthetic active interval allowing us to use the simulator for biological experiments under radiation and atmospheric conditions close as much as possible to those expected on extrasolar planets. The simulator is a laboratory tool which is part of the "Atmosphere in a Test Tube" project, aimed to study the photosynthetic efficiency of bacteria under alien environmental conditions and their possible impact on the atmosphere of the host planet. This paper describes the software developed to control the simulator. We begin by presenting a conceptual overview of the instruments and then illustrating the top-level requirements and the architecture of the control software. Finally, we give a description of the graphical user interface

Atmospheres in a Test Tube: state of the art at the Astronomical Observatory of Padova.

At the Astronomical observatory of Padova we are trying to answer some questions about the detectability of biosignatures in the exoplanetary atmospheres, working in the framework of the project Atmosphere in a Test Tube. In particular we are investigating how the presence of photosynthetic biota living on the surface of a planet orbiting in the HZ of an M type star may modify the atmospheric gas abundances. This can be achieved in laboratory with an environmental simulator called MINI - LISA. The simulator allows to modify the temperature and the pressure inside a test chamber, where a selected population of photosynthetic bacteria is arranged. We'll focalize our experiments on the following bacteria: Acaryochloris marina, Halomicronema hongdechloris, Leptolyngbya sp.1 and Chlorogloeopsis fritschii. The first two bacteria are naturally provided with NIR light metabolizers, like Chl-d and Chl-f, while the last two can develop such pigments if grown in NIR light. The experiment will lead us to obtain useful data to be compared with the ones expected either by the future space missions (JWST, ARIEL) and ground based new instrumentation (SPHERE@VLT; GPI@GEMINI; PCS@E-ELT). In this talk we discuss the layout of the experiment and its state of art