Insight into the management of actinic keratosis: a qualitative interview study among general practitioners and dermatologists

_Background_ The increasing incidence of actinic keratosis (AK) is causing a large burden on healthcare systems. The current management of patients with AK seems to vary within and between primary and secondary care; however, an in-depth understanding of healthcare providers’ management of AK is currently lacking. Objectives To gain insight into the management of AK by exploring the underlying motives of current practices among general practitioners (GPs) and dermatologists in the Netherlands. _Methods_ A qualitative study was conducted consisting of semistructured individual interviews with 22 GPs and 18 dermatologists focusing on the underlying motives regarding AK management. A predefined topic list was used. All interviews were audiotaped, transcribed verbatim and inductively analysed by two researchers drawing on elements of grounded theory. _Results_ GPs reported conducting limited proactive clinical assessments of cutaneous photodamage due to a perceived lack of value, varying in their method of diagnosing AK. They mainly applied cryotherapy or referred to secondary care due to lack of experience, varying in their applications and providing mostly patient-driven follow-up care. They also reported a great need for guidelines due to a lack of knowledge of AK management. Dermatologists indicated pursuing proactive clinical assessments of cutaneous photodamage and the goal of providing guideline-driven AK care. However, patient preferences still largely influence both treatment choices and follow-up regimens. Furthermore, dermatologists reported the need to improve AK and skin cancer management in primary care. _Conclusions_ For AK care to become more standardized and uniform in Dutch primary care, the implementation of guidelines and (continuing) education are needed to address the commonly reported barriers of lack of value, experience and knowledge among GPs. For efficient use of care among dermatologists, shared decision-making tools along with adequate (framing of) patient information may be useful

Influenza at the animal–human interface: a review of the literature for virological evidence of human infection with swine or avian influenza viruses other than A(H5N1)

Factors that trigger human infection with animal influenza virus progressing into a pandemic are poorly understood. Within a project developing an evidence-based risk assessment framework for influenza viruses in animals, we conducted a review of the literature for evidence of human infection with animal influenza viruses by diagnostic methods used. The review covering Medline, Embase, SciSearch and CabAbstracts yielded 6,955 articles, of which we retained 89; for influenza A(H5N1) and A(H7N9), the official case counts of the World Health Organization were used. An additional 30 studies were included by scanning the reference lists. Here, we present the findings for confirmed infections with virological evidence. We found reports of 1,419 naturally infected human cases, of which 648 were associated with avian influenza virus (AIV) A(H5N1), 375 with other AIV subtypes, and 396 with swine influenza virus (SIV). Human cases naturally infected with AIV spanned haemagglutinin subtypes H5, H6, H7, H9 and H10. SIV cases were associated with endemic SIV of H1 and H3 subtype descending from North American and Eurasian SIV lineages and various reassortants thereof. Direct exposure to birds or swine was the most likely source of infection for the cases with available information on exposure

Promising Neighbourhoods

Meta data research project Promising Neighbourhood

Promising Neighbourhoods

Meta data research project Promising Neighbourhood

Recapitulating infection, thermal sensitivity and antiviral treatment of seasonal coronaviruses in human airway organoids

This research project aims to discover the impact of temperature on seasonal coronavirus propagation and identify the virus-host interactions upon seasonal coronavirus 229E and OC43 infection

Recapitulating infection, thermal sensitivity and antiviral treatment of seasonal coronaviruses in human airway organoids

This research project aims to discover the impact of temperature on seasonal coronavirus propagation and identify the virus-host interactions upon seasonal coronavirus 229E and OC43 infection

Monkeypox virus infection in human kidney organoids

Human kidney organoids were inoculated with monkeypox virus (MPXV), samples at 1 (MPXV1H), 48 (MPXV48H), 96 (MPXV96H) hours and 7 (MPXV7D) days post-inoculation were harvested, and organoids cultured 7 days without infection was included as a negative control. There are 4 replicates for each group. These samples were subjected to genome-wide RNA sequencing. These are the data of RNA-seq on transcriptome

Detection of fusion transcripts and their genomic breakpoints from RNA sequencing data - Table S03 - All detected SVs.xlsx

Large concatenated results table on all samples of the Dr. Disco study

The EGFRvIII Transcriptome in glioblastoma - public data repository

Compiled dataset from a large omics EGFRvIII study

The EGFRvIII Transcriptome in glioblastoma - public data repository

Compiled dataset from a large omics EGFRvIII study