Cell separation using electric fields

The present invention involves methods and devices which enable discrete objects having a conducting inner core, surrounded by a dielectric membrane to be selectively inactivated by electric fields via irreversible breakdown of their dielectric membrane. One important application of the invention is in the selection, purification, and/or purging of desired or undesired biological cells from cell suspensions. According to the invention, electric fields can be utilized to selectively inactivate and render non-viable particular subpopulations of cells in a suspension, while not adversely affecting other desired subpopulations. According to the inventive methods, the cells can be selected on the basis of intrinsic or induced differences in a characteristic electroporation threshold, which can depend, for example, on a difference in cell size and/or critical dielectric membrane breakdown voltage. The invention enables effective cell separation without the need to employ undesirable exogenous agents, such as toxins or antibodies. The inventive method also enables relatively rapid cell separation involving a relatively low degree of trauma or modification to the selected, desired cells. The inventive method has a variety of potential applications in clinical medicine, research, etc., with two of the more important foreseeable applications being stem cell enrichment/isolation, and cancer cell purging

Rate Measurement of D0→K+π−π0D^{0}\to K^{+}\pi^{-}\pi^{0} and Constraints on D0−D0‾D^{0} - \overline{D^{0}} Mixing

We present an observation and rate measurement of the decay D0 -> K+pi-pi0 produced in 9/fb of e+e- collisions near the Upsilon(4S) resonance. The signal is inconsistent with an upward fluctuation of the background by 4.9 standard deviations. We measured the rate of D0 -> K+pi-pi0 normalized to the rate of D0bar -> K+pi-pi0 to be 0.0043 +0.0011 -0.0010 (stat) +/- 0.0007 (syst). This decay can be produced by doubly-Cabibbo-suppressed decays or by the D0 evolving into a D0bar through mixing, followed by a Cabibbo-favored decay to K+pi-pi0. We also found the CP asymmetry A=(8 +25 -22)% to be consistent with zero.Comment: 10 pages postscript, also available through http://w4.lns.cornell.edu/public/CLN

First Observation of barB0 to D*0 pi+pi+pi-pi- Decays

We report on the observation of B0bar -> D*0 pi+ pi+ pi- pi- decays. The branching ratio is (0.30 +/- 0.07 +/- 0.06)%. Interest in this particular mode was sparked by Ligeti, Luke and Wise who propose it as a way to check the validity of factorization tests in B0bar -> D*+ pi+ pi- pi- pi0 decays.Comment: 11 pages postscript, also available through http://w4.lns.cornell.edu/public/CLNS, Version to appear in Phys. Rev.

A Search for Charmless B→VVB\to VV Decays

We have studied two-body charmless decays of the $B$ meson into the final states $\rho^0 \rho^0$, $K^{*0} \rho^0$, $K^{*0} K^{*0}$, $K^{*0} \bar{K^{*0}}$, $K^{*+} \rho^0$, $K^{*+} \bar{K^{*0}}$, and $K^{*+} K^{*-}$ using only decay modes with charged daughter particles. Using 9.7 million $B \bar{B}$ pairs collected with the CLEO detector, we place 90% confidence level upper limits on the branching fractions, $(0.46-7.0)\times 10^{-5}$, depending on final state and polarization.Comment: 8 pages postscript, also available through http://w4.lns.cornell.edu/public/CLN

Search for a Scalar Bottom Quark with Mass 3.5-4.5 GeV/c2c^{2}

We report on a search for a supersymmetric $\tilde{B}$ meson with mass between 3.5 and 4.5 GeV/$c^2$ using 4.52 ${\rm fb}^{-1}$ of integrated luminosity produced at $\sqrt{s}=10.52$ GeV, just below the $e^+e^-\to B\bar{B}$ threshold, and collected with the CLEO detector. We find no evidence for a light scalar bottom quark.Comment: 10 pages postscript, also available through http://w4.lns.cornell.edu/public/CLN

Observation of New States Decaying into Λc+π−π+\Lambda_{c}^{+}\pi^{-}\pi^{+}

Using 13.7 fb^{-1} of data recorded by the CLEO detector at CESR, we investigate the spectrum of charmed baryons which decay into Lambda_c^+ pi^- pi^+ and are more massive than the Lambda_{c1} baryons. We find evidence for two new states: one is broad and has an invariant mass roughly 480 MeV above that of the Lambda_c^+; the other is narrow with an invariant mass of 596 +- 1 +- 2 MeV above the Lambda_c^+ mass. These results are preliminary.Comment: 11 pages postscript, also available through http://w4.lns.cornell.edu/public/CLN

Correlated /\c-/\cbar production in e+e- annihilations at sqrt{s}~10.5 GeV

Using 13.6/fb of continuum two-jet e+e- -> ccbar events collected with the CLEO detector, we have searched for baryon number correlations at the primary quark level. We have measured the likelihood for a /\c+ charmed baryon to be produced in the hemisphere opposite a /\c- relative to the likelihood for a /\c+ charmed baryon to be produced opposite an anticharmed meson Dbar; in all cases, the reconstructed hadrons must have momentum greater than 2.3 GeV/c. We find that, given a /\c- (reconstructed in five different decay modes), a /\c+ is observed in the opposite hemisphere (0.72+/-0.11)% of the time (not corrected for efficiency). By contrast, given a Dbar in one hemisphere, a /\c+ is observed in the opposite hemisphere only (0.21+/-0.02)% of the time. Normalized to the total number of either /\c- or Dbar ``tags'', it is therefore 3.52+/-0.45+/-0.42 times more likely to find a /\c+ opposite a /\c- than a Dbar meson. This enhancement is not observed in the JETSET 7.3 e+e- -> ccbar Monte Carlo simulation.Comment: 19 pages, Latex, one figure separat

Hadronic Mass Moments in Inclusive Semileptonic B Meson Decays

We have measured the first and second moments of the hadronic mass-squared distribution in B -> X_c l nu, for P(lepton) > 1.5 GeV/c. We find <M_X^2 - M_D[Bar]^2> = 0.251 +- 0.066 GeV^2, )^2 > = 0.576 +- 0.170 GeV^4, where M_D[Bar] is the spin-averaged D meson mass. From that first moment and the first moment of the photon energy spectrum in b -> s gamma, we find the HQET parameter lambda_1 (MS[Bar], to order 1/M^3 and beta_0 alpha_s^2) to be -0.24 +- 0.11 GeV^2. Using these first moments and the B semileptonic width, and assuming parton-hadron duality, we obtain |V_cb| = 0.0404 +- 0.0013.Comment: 11 pages postscript, also available through http://w4.lns.cornell.edu/public/CLNS, submitted to PR

Bounds on the CP Asymmetry in Like-Sign Dileptons from B0Bˉ0B^{0}\bar{B}^{0} Meson Decays

We have measured the charge asymmetry in like-sign dilepton yields from B^0 B^0-bar meson decays using the CLEO detector at the Cornell Electron Storage Ring. We find a_ll = [N(l+l+) - N(l-l-)]/[N(l+l+) + N[l-l-)] = +0.013 +/- 0.050 +/- 0.005 . We combine this result with a previous, independent measurement and obtain Re(epsilon_B)/(1+|epsilon_B|^2) = +0.0035 +/- 0.0103 +/- 0.0015 (uncertainties are statistical and systematic, respectively) for the CP impurity parameter, epsilon_B.Comment: 11 pages postscript, also available through http://w4.lns.cornell.edu/public/CLN