A unique maternal and placental galectin signature upon SARS-CoV-2 infection suggests galectin-1 as a key alarmin at the maternal–fetal interface

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic imposed a risk of infection and disease in pregnant women and neonates. Successful pregnancy requires a fine-tuned regulation of the maternal immune system to accommodate the growing fetus and to protect the mother from infection. Galectins, a family of β-galactoside–binding proteins, modulate immune and inflammatory processes and have been recognized as critical factors in reproductive orchestration, including maternal immune adaptation in pregnancy. Pregnancy-specific glycoprotein 1 (PSG1) is a recently identified gal-1 ligand at the maternal–fetal interface, which may facilitate a successful pregnancy. Several studies suggest that galectins are involved in the immune response in SARS-CoV-2–infected patients. However, the galectins and PSG1 signature upon SARS-CoV-2 infection and vaccination during pregnancy remain unclear. In the present study, we examined the maternal circulating levels of galectins (gal-1, gal-3, gal-7, and gal-9) and PSG1 in pregnant women infected with SARS-CoV-2 before vaccination or uninfected women who were vaccinated against SARS-CoV-2 and correlated their expression with different pregnancy parameters. SARS-CoV-2 infection or vaccination during pregnancy provoked an increase in maternal gal-1 circulating levels. On the other hand, levels of PSG1 were only augmented upon SARS-CoV-2 infection. A healthy pregnancy is associated with a positive correlation between gal-1 concentrations and gal-3 or gal-9; however, no correlation was observed between these lectins during SARS-CoV-2 infection. Transcriptome analysis of the placenta showed that gal-1, gal-3, and several PSG and glycoenzymes responsible for the synthesis of gal-1-binding glycotopes (such as linkage-specific N-acetyl-glucosaminyltransferases (MGATs)) are upregulated in pregnant women infected with SARS-CoV-2. Collectively, our findings identify a dynamically regulated “galectin-specific signature” that accompanies the SARS-CoV-2 infection and vaccination in pregnancy, and they highlight a potentially significant role for gal-1 as a key pregnancy protective alarmin during virus infection

Noise Analysis of Open-Loop and Closed-Loop Readout Systems for Phase Sensitive Magnetic Field Sensors

International audienceransmission surface acoustic wave (SAW) sensors are widely used in various fields of application. In order to maximize the limit of detection (LOD) of such sensor systems, it is of high importance to understand and to be able to quantify the relevant noise sources. In this paper, low noise readout systems for the application with a SAW delay line magnetic field sensor in an open-loop and closed-loop configuration are presented and analyzed with regard to their phase noise contribution. By applying oscillator phase noise theory to closed-loop sensor systems, it is shown that the phase noise of the SAW delay line oscillator can be predicted accurately. This allows the derivation of expressions for the limits of detection for both readout structures. Based on these equations, the equivalence between the LOD of open-loop and closed-loop SAW delay line readout can be shown analytically, assuming that the sensor contributes the dominant phase noise. This equality is verified by measurements. These results are applicable to all kinds of phase sensitive delay line sensors

Noise Analysis and Comparison of Phase-and Frequency-Detecting Readout Systems: Application to SAW Delay Line Magnetic Field Sensor

International audienceTransmission surface acoustic wave (SAW) sensors are widely used in various fields of application. In order to improve the limit of detection (LOD) of such sensor systems, it is essential to understand and quantify the relevant noise sources. Only then, strategies for noise reduction can be developed. In this paper, low noise readout systems for the application with SAW sensors in an open-loop and a closed-loop configuration are presented and experimentally investigated with regard to their phase noise on the example of a SAW delay line magnetic field sensor. Besides a comprehensive analysis of the phaseand frequency modulated signals, respectively, previously derived equations in a theoretic study for describing the LOD of both readout structures are utilized in the experimental context. According to the theory, the same LOD is also obtained in the experiment for all frequencies for which the noise contributions of the readout electronics are negligible. To the best of our knowledge, this is the first experimental study that directly compares both operating modes for the same sensor and in terms of the overall achievable LOD. The results are applicable to all kinds of phase-sensitive delay-line sensors