1 research outputs found
Tuning the Cell-Adhesive Properties of Two-Component Hybrid Hydrogels to Modulate Cancer Cell Behavior, Metastasis, and Death Pathways
This work presents a polysaccharide
and protein-based
two-component
hybrid hydrogel integrating the cell-adhesive gelatin-tyramine (G-Tyr)
and nonadhesive hyaluronic acid-tyramine (HA-Tyr) through enzyme-mediated
oxidative coupling reaction. The resulting HA-Tyr/G-Tyr hydrogel reflects
the precise chemical and mechanical features of the cancer extracellular
matrix and is able to tune cancer cell adhesion upon switching the
component ratio. The cells form quasi-spheroids on HA-Tyr rich hydrogels,
while they tend to form an invasive monolayer culture on G-Tyr rich
hydrogels. The metastatic genotype of colorectal adenocarcinoma cells
(HT-29) increases on G-Tyr rich hydrogels which is driven by the material’s
adhesive property, and additionally confirmed by the suppressed gene
expressions of apoptosis and autophagy. On the other hand, HA-Tyr
rich hydrogels lead the cells to necrotic death via oxidative stress
in quasi-spheroids. This work demonstrates the ideality of HA-Tyr/G-Tyr
to modulate cancer cell adhesion, which also has potential in preventing
primary metastasis after onco-surgery, biomaterials-based cancer research,
and drug testing