15 research outputs found
Extracorporeal Photopheresis: Effective Therapy for Steroid Dependent and Refractory Acute Graft-Versus-Host Disease
Allogeneic Stem Cell Transplantation for FLT3/ITD Positive Acute Myeloid Leukemia in CR1: Implication for Personalized Therapy to Prevent Early Relapses
Extracorporeal Photopheresis: Effective Therapy for Steroid Dependent and Refractory Acute Graft-Versus-Host Disease
Genetic Variation in Donor CTLA-4 Regulatory Region Is Associated With Relapsed Free Survival and Overall Survival After Allogenieic Hematopoietic Cell Transplant
Cytomegalovirus (CMV) Epitope–Specific CD4 + T Cells Are Inflated in HIV + CMV + Subjects
Select CMV epitopes drive life-long CD8+ T cell memory inflation, but the extent of CD4 memory inflation is poorly studied. CD4+ T cells specific for human CMV (HCMV) are elevated in HIV+ HCMV+ subjects. To determine whether HCMV epitope–specific CD4+ T cell memory inflation occurs during HIV infection, we used HLA-DR7 (DRB1*07:01) tetramers loaded with the glycoprotein B DYSNTHSTRYV (DYS) epitope to characterize circulating CD4+ T cells in coinfected HLA-DR7+ long-term nonprogressor HIV subjects with undetectable HCMV plasma viremia. DYS-specific CD4+ T cells were inflated among these HIV+ subjects compared with those from an HIV− HCMV+ HLA-DR7+ cohort or with HLA-DR7–restricted CD4+ T cells from the HIV-coinfected cohort that were specific for epitopes of HCMV phosphoprotein-65, tetanus toxoid precursor, EBV nuclear Ag 2, or HIV gag protein. Inflated DYS-specific CD4+ T cells consisted of effector memory or effector memory–RA+ subsets with restricted TCRβ usage and nearly monoclonal CDR3 containing novel conserved amino acids. Expression of this near-monoclonal TCR in a Jurkat cell–transfection system validated fine DYS specificity. Inflated cells were polyfunctional, not senescent, and displayed high ex vivo levels of granzyme B, CX3CR1, CD38, or HLA-DR but less often coexpressed CD38+ and HLA-DR+. The inflation mechanism did not involve apoptosis suppression, increased proliferation, or HIV gag cross-reactivity. Instead, the findings suggest that intermittent or chronic expression of epitopes, such as DYS, drive inflation of activated CD4+ T cells that home to endothelial cells and have the potential to mediate cytotoxicity and vascular disease