The Effect of Phosphorus and Potassium on the Growth and Quality of Viola (Viola cornuta L.) Seed Production

Viola cornuta L. is an ornamental plant that is primarily propagated by high-quality seeds that have high demand in subtropical countries. The use of fertilizers according to recommendations results in the growth of parent plants below the standard. Furthermore, seed production is generated below 2 g per plant and the seed germination capacity is below 80%. In addition, phosphorus (P) and potassium (K) nutrients can increase the growth and seed production of the plants, including ornamental plants. The study aimed to determine the influence of P and K nutrients on growth and seed production of V. cornuta L. Research was carried out in November 2018 - January 2019 at PT. Selektani Horticulture, Ngablak, Magelang. There were seven treatments with four replications. Experiments were arranged according to the Randomized Complete Block Design (RCBD). Plants were grown in 5l volume-polybags. Fertilization was done by watering the planting media. Analysis of variance was performed at 95% accuracy. The study shows that P and K nutrients have a highly significant effect on height, number of flowers, number of seed per capsule, seed production and germination energy and significantly affected the number of leaves. The addition of both P and K fertilizers in the vegetative and P in the generative stage (P6) can result in the productivity of 3.28 g plant-1 and produce germination energy ≥90%. It is recommended for increasing growth and seed production

Surat Keterangan Uji Etical Clearence No : 2934/UN3.1.6/KP/2022 tentang Penelitian dengan Judul Protein Profile of Sporozoite of Leucocytozoon sp. from Culicoides sp.

Kumpulan Surat SK Endang Suprihati yang dipergunakan untuk Keperluan Kenaikan Pangkat dan lain-lai

Evaluation of The Antigenicity and Immunogenicity of Eimeria tenella by Reproductive Index and Histopathological Changes of Cecal Coccidiosis Virulent Live Vaccine in Broiler Chickens

Background: The development of vaccine to control coccidiosis caused by Eimeria tenella (E. tenella) in chickens is intensifying because of the increasing threat of drug resistance to anticoccidial agents. It is important, therefore, to develop a reliable standard method for the assessment of vaccine afficacy particularly antigenicity and immunogenicity become crucial. Evaluation of E. tenella antigenicity and immunogenicity to some low doses can be reflected by reproductive index and histopathological changes. Materials and Methods: The complete random design of research was used in this study. Sixty of two weeks old broilers were divided into four groups and each group composed 15 replications. The group 1 was chicken group without virulent E. tenella oocyst inoculation. The group 2, 3 and group 4 were chicken group inoculated with virulent E. tenella oocyst at doses of 1.0 x 102, 2.0 x 102, 3.0 x 102, respectively. Then all chicken groups were challenged with E. tenella oocyst at doses of 1.0 x 103. Observation of research that represented antigenicity and immunogenicity was clinical sign, reproductive index, histopathological changes. Results: On virulent E. tenella inoculation step, some clinical signs such as appetite, weakness, and diarrhea were very slight on all chicken groups. While on challenge test step, there were no clinical signs of all chicken groups except the group 1. For the reproductive index of virulent E. tenella inoculation step, there were no significantly differences in all chicken groups except the group 1. As reproductive index, the same result pattern was seen for histopathological changes. Conclusion: The low number virulent E. tenella had low reproductive index and few histopathological changes effect that represents a promising strategy to prevent cecal coccidiosis in chickens. Key words: antigenicity, E.tenella, histopthological changes, immunogenicity, reproductive inde

Surat Keterangan Uji Etical Clearence No : 2934/UN3.1.6/KP/2022 tentang Penelitian dengan Judul Uji Reaktivitas Protein 30 kDA Bakteri Aeromonas hydrophila yang Diisolasi dari Ikan Air Tawar dengan Teknik Indirect ELISA

Kumpulan Surat SK Endang Suprihati yang dipergunakan untuk Keperluan Kenaikan Pangkat dan lain-lai

Surat Keterangan Uji Etical Clearence No : 2935/UN3.1.6/KP/2022 tentang Penelitian dengan Judul Reproductive Characteristic of a Precocious Line of E. tenella Sporozoite as Material Bioactive in Embyionating Eggs and the Implications of the Findings Appraised

Kumpulan Surat SK Endang Suprihati yang dipergunakan untuk Keperluan Kenaikan Pangkat dan lain-lai

Surat Keterangan Uji Etical Clearence No : 2935/UN3.1.6/KP/2022 tentang Penelitian dengan Judul Vaksininasi Protein Ekskretori-Sekretori Toxoplasma gondii Hasil Biakan in vivo Membangkitkan Respons Imun Non Protektif

Kumpulan Surat SK Endang Suprihati yang dipergunakan untuk Keperluan Kenaikan Pangkat dan lain-lai

Surat Keterangan Uji Etical Clearence No : 2934/UN3.1.6/KP/2022 tentang Penelitian dengan Judul Eksplorasi Protein Antigenik Leucocytozoon caulleryi sebagai Kit Diagnostik Leucocytozoonosis pada Ayam Broiler

Kumpulan Surat SK Endang Suprihati yang dipergunakan untuk Keperluan Kenaikan Pangkat dan lain-lai

Assessment of Profile of Antigenicity and Immunogeniticity of Eimeria maxima and Eimeria tenella through Propagative Level and Histopathological Changes in Site Infection for Exploration of Optimal Low Doses of Bivalent Chicken Coccidiosis Live Vaccine

Live vaccines group was the first to be studied, due to the fact that live parasites reduce further reinfection. The development of vaccine to control coccidiosis caused by E. maxima and E. tenella in chickens is intensifying because of the increasing threat of drug resistance to anticoccidial agents. It is important, therefore, to develop a reliable standard method for the assessment of vaccine afficacy particularly antigenicity and immunogenicity become crucial. Evaluation of profile of antigenicity and immunogenicity of E. maxima and E. tenella to some low doses can be reflected through propagative level and histopathological changes. The complete random design of research was used in this study. Sixty of two weeks old broilers were divided into four groups and each group composed 15 replications. The group 1 and group 2 were chicken group inoculated orally 5 x 102 and 1 x 103 virulent E. maxima oocysts, respectively. The group 3 and group 4 were chicken group inoculated with 2.5 x 102 and 5 x 102 virulent E. tenella oocysts, respectively. Then all chickens of group 1 and group 2 were challenged with E. maxima oocyst at doses of 5 x 103, while chicken group 3 and group 4 were challenged with E. tenella oocyst at doses of 2.5 x 103. Observation of research that represented antigenicity and immunogenicity was clinical sign, propagative level, histopathological changes. On primary inoculation, chicken group 1 and 3 which low doses of E. maxima and E. tenella respectively inoculated was some clinical signs such as appetite, weakness, and diarrhea were not seen on all chicken, low parasite propagative level and few histopathological changes on development site, whereas on group 2 and 4 which higher doses of E. maxima and E. tenella respectively inoculated was appeared clinical signs such as appetite, weakness, and diarrhea, moderate parasite propagative level and histopathological changes on development site. Then on challenge test, group 1 and 2 which challenged E. maxima and group 3 and 4 which challenged E. tenella showed there were no clinical signs such as appetite, weakness, and diarrhea on all chicken groups. For the propagative level and histopathological changes, there were no significantly differences in all chicken groups. We concluded that the low doses of virulent E. maxima and E. tenella had low propagative level and few histopathological changes effect that represents a promising strategy to prevent chickens coccidiosis particularly both species. Keywords: antigenicity, E.tenella, E. maxima, histopthological changes, immunogenicity, propagative leve

Surat Keterangan Uji Etical Clearence No : 2934/UN3.1.6/KP/2022 tentang Penelitian dengan Judul Histopathological studies on Leucocytozoon Caulleryi Infection on Broiler in Endemic Area of Indonesia

Kumpulan Surat SK Endang Suprihati yang dipergunakan untuk Keperluan Kenaikan Pangkat dan lain-lai

Surat Keterangan Uji Etical Clearence No : 2934/UN3.1.6/KP/2022 tentang Penelitian dengan Judul Antigenic Protein of Leucocytozoon caulleryi schizont Inducing Cellular Immune Response: TLR-2 and CD4 as Marker

Kumpulan Surat SK Endang Suprihati yang dipergunakan untuk Keperluan Kenaikan Pangkat dan lain-lai