Fabrication of porcine DNA biosensor based on ruthenium bipyridine complex

Electrochemical DNA biosensor for detection of porcine oligonucleotides based on ruthenium (II) as label redox complex has been developed. The system of biosensor is based on the modified screen printed carbon electrode (SPE) with goldnanoparticles (AuNPs) as transducer immobilized with poly(n-butylacrylate-N-acryloxysuccinimide) microsphere and porcine DNA probe sequences was attached onto it via the covalent bond. The ruthenium(II) complex, [Ru(bpy)2PIP]2+ PIP = 2-phenylimidazo[4,5-f[1,10-phenanthroline] intercalator has been been used to determine prcine DNA. The biosensor was measured and optimized by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The interaction of [Ru(bpy)2PIP]2+ with various DNA with different sequences, i) single stranded probe DNA, ii) after hybridization with its complementary DNA and iii) after hybridization with mismatch complementary DNA were studied. The results indicated that the interaction of [Ru(bpy)2PIP]2+ with hybridized complementary DNA gave the highest response. Thus, development of porcine DNA biosensor and influences of many factors such as [Ru(bpy)2PIP]2+ complex and DNA probe concentration, DNA probe immobilization and hybridization time, pH, ionic strength, buffer concentration and temperatures were also studied to evaluate the performance of biosensor. The concentration of [Ru(bpy)2PIP]2+ complex and DNA probe were found to be optimal at 50 µM and 2 µM, respectively. The optimal time for DNA probe immobilization and hybridization were 7 hours and 60 minutes, accordingly. The optimal condition of pH, ionic strength, buffer concentration and temperatures were at pH 7.0, 1.0 M NaCl, 0.05 M of Na-phosphate buffer and 25 °C, respectively. The linear range of different concentration complementary DNA was within the range between 1.0 x 10-13 M to 1.0 x 10-8 M. This study was first reported the used of [Ru(bpy)2PIP]2+ for detection of porcine oligonucleotides

Crystal structure, hirshfeld analysis and DFT study of 6,7-dimethoxy-3-[(5-chloro-1,3,3-trimethylindolin-2-ylidene)methyl]isobenzofuran-1(3h)-one

The coupling reaction of 5-chloro-1,3,3-methylenindoline with 6-formyl-2,3-dimethoxy benzoic acid under solvent-free microwave irradiation gave of 6,7-dimethoxy-3-[(5-chloro-1,3,3-trimethylindolin-2-ylidene)methyl]isobenzofuran- 1(3H)-one, C22H22ClNO4 in high yield. The molecular structure consists of indoline system (C1-C8/N1) and isobenzofuran system (C10-C17/O1) connected by the enamine carbon. The title compound is crystallised in P21/n space group, Z’=4 with two independent molecules in centrosymmetric dimer. By using Hirshfeld surface and two-dimensional fingerprint plots, the presence of short intermolecular interactions and π-π stacking in the crystal structure were analysed. The DFT study is performed by comparing the calculation data with the X-ray data and analysing the HOMO and LUMO value, which gave -0.26952 a.u. and -0.17760 a.u., respectively

A comparative study of microwave-assisted and conventional heating methods of the synthesis of 1-(Naphthalene-1-Yl)-3-(O, M, P-Tolyl)thioureas, DFT analysis, antibacterial evaluation and drug-likeness assessment

New isomers of naphthalene-monothioureas, 3a-3c, were synthesized and characterized by using infrared (IR), 1 H and 13 C nuclear magnetic resonance (NMR) and mass spectroscopies (MS). The compounds were obtained from the reaction of o-, m-, p- toluidine with naphthyl isothiocyanate using conventional reflux and microwave-assisted irradiation methods. Microwave-assisted reaction gave higher yields with reduces the reaction time compared to the conventional heating method. Density Functional Theory (DFT) calculations with 6-31G(d, p) basic set at the B3LYP level is carried out to optimize the structure of isomers and calculate the IR, 1 H and 13 C NMR spectra. The DFT B3LYP calculation of FTIR, 1 H and 13C NMR spectra of 3a-3c isomers were in accordance with the experimental data. The calculation of frontier molecular orbitals was also selected to study a relationship as a molecular descriptor in antibacterial activity. The isomers were screened for their in vitro antibacterial activity by using Staphylococcus aureus bacteria. Inhibition activity was displayed for compound 3c with the value of inhibition zone 7 ± 0.34 mm at a concentration of 10 µg/mL. All compounds appeared to be in agreement with Lipinski’s rule of five assessments as they adhered to most of the rules that can be preliminarily classified as active drug-like

A New Thiourea Compound as Potential Ionophore for Metal Ion Sensor

A new thiourea compound, 2,2-oxybis(ethyl)-4-(1-naphthyl)-3-thiourea 3 has been synthesized and characterized by using FTIR, 1H-NMR, 13C-NMR, and MS spectroscopy. The binding properties of with various cations were also carried out using ‘naked eye’, UV-vis and 1H-NMR titration experiments. This compound exhibited effective binding for Hg2+ in the presence of other cations, such as Ag+, Ni2+, Sn2+, Zn2+, Fe2+, Cu2+, and Pb2+. Continuous variation titration experiments were conducted in order to determine the binding behavior for Hg2+. Stoichiometry of the host and guest binding interactions were also determined using continuous variation titration experiments and plotting molar-ratio curves. Pearson Product moment method was employed to calculate the correlation coefficient, and non-linear regression equation was used to calculate dissociation constant Kd. Molar-ratio and binding constant data substantiated the presence of binding sites for the compound 3