PCoA and NMDS.

<p>PCoA (Fig. A and B) and MNDS (Fig. C and D) showing the representation of vectorial analysis of sequences found in feces of healthy horses (blue dots) and horses affected by colitis (red dots). Results were obtained using the Yue & Clayton measure (Fig. A and C), the Jaccard index (Fig. B and D).</p

Classification and species richness of the fecal bacteria of healthy horses and horses affected by colitis.

<p>Classification of the intestinal bacteria of healthy horses and horses affected by colitis (in percentages) at the phylum level, total number of bacterial reads and species richness after data cleaning (pyrosequencing noise and chimera removal) and filtering (e-value of 30, minimum identity of 97% and minimum alignment of 75bp on MG-RAST).</p

Pyrosequencing metrics of the cleaned data and its distribution at the Kingdom level.

<p>Total number of reads after data cleaning (pyrosequencing noise and chimera removal), after filtering (e-value of 30, minimum identity of 97% and minimum alignment of 75bp on MG-RAST), and percentage of reads classified by MG-RAST using the SSU databank as Bacteria, Eukaryota, Archaea, unclassified bacteria and sequences unassigned to any Kingdom. Means and standard deviations (±SD) among healthy horses and horses with colitis are also presented.</p

Pyrosequencing metrics from raw data before and after standard MG-RAST quality control (QC) filters.

<p>Total number of sequences, number of base pairs and the mean length of sequences (bp) present in the original fasta file before and after MG-RAST standard quality control filters. Means and standard deviations (±SD) among healthy horses and horses with colitis are also presented.</p

Total number of sequences, coverage, number of Phylotypes and inverted Simpson with lower and upper confidence interval limits.

*<p>Phylotypes.</p

Phylogenetic trees – Phylotypes approach.

<p>Phylogenetic tree demonstrating the similarity of Phylotypes found in feces of healthy horses (Healthy 1–6) and horses affected by colitis (Colitis 1–10). Results were obtained using the Yue & Clayton measure (A) and the Jaccard index (B).</p

Total number of sequences, coverage, number of OTUs and inverted Simpson with lower and upper confidence interval limits.

<p>Total number of sequences, coverage, number of OTUs and inverted Simpson with lower and upper confidence interval limits.</p

Phylogenetic trees – OTUs approach.

<p>Phylogenetic tree demonstrating the similarity of OTUs found in feces of healthy horses (Healthy 1–6) and horses affected by colitis (Colitis 1–10). Results were obtained using the Yue & Clayton measure (A) and the Jaccard index (B).</p

Fecal bacterial population.

<p>Overall percentages of bacterial populations at the phylum level (Fig. A) and intra-phylum variation (Fig. B) present in feces of healthy horses and horses affected by colitis.</p