8 research outputs found
Kappa scores and agreement for the rK39 RDT, rK39 ELISA, qPCR, and <i>Leishmania</i> antigen ELISA in 96 people living with HIV (PLHIV) with asymptomatic <i>Leishmania</i> infection (ALI) in Bihar, India.
Kappa scores and agreement for the rK39 RDT, rK39 ELISA, qPCR, and Leishmania antigen ELISA in 96 people living with HIV (PLHIV) with asymptomatic Leishmania infection (ALI) in Bihar, India.</p
Multivariable risk factor analysis for ALI in PLHIV including the <i>Leishmania</i> antigen ELISA in addition to qPCR, rK39 ELISA and RDT in the definition of ALI.
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Baseline viral load in 1,296 people living with HIV (PLHIV) with asymptomatic <i>Leishmania</i> infection (ALI) in Bihar, India.
Baseline viral load in 1,296 people living with HIV (PLHIV) with asymptomatic Leishmania infection (ALI) in Bihar, India.</p
Prevalence of asymptomatic <i>Leishmania</i> infection (ALI) in 1,296 people living with HIV (PLHIV) in Bihar, India by serological, molecular, and antigen detection methods.
Prevalence of asymptomatic Leishmania infection (ALI) in 1,296 people living with HIV (PLHIV) in Bihar, India by serological, molecular, and antigen detection methods.</p
Comparison of baseline clinical parameters and haematology results in 1,296 people living with HIV (PLHIV) with and without asymptomatic <i>Leishmania</i> infection (ALI) in Bihar, India.
Comparison of baseline clinical parameters and haematology results in 1,296 people living with HIV (PLHIV) with and without asymptomatic Leishmania infection (ALI) in Bihar, India.</p
Household related risk factors for asymptomatic <i>Leishmania</i> infection (ALI) in PLHIV including the <i>Leishmania</i> antigen ELISA in addition to qPCR, rK39 ELISA and RDT in the definition of ALI.
(DOCX)</p
HIV-related risk factors for asymptomatic <i>Leishmania</i> infection (ALI) in 1,296 people living with HIV (PLHIV) in Bihar, India.
HIV-related risk factors for asymptomatic Leishmania infection (ALI) in 1,296 people living with HIV (PLHIV) in Bihar, India.</p
Additional file 1 of Genetic analysis and molecular basis of G6PD deficiency among malaria patients in Thailand: implications for safe use of 8-aminoquinolines
Additional file 1: Figure S1. Primers used in (A) multiplex HRM for the detection of 15 G6PD mutations and (B) G6PD gene sequencing. Table S1. Primers used in multiplex HRM assays. Table S2. Primers used for site-directed mutagenesis. Figure S2. The frequency distribution of G6PD enzyme activity in (A) males and (B) females. Figure S3. Box plot of G6PD activity for each variant among (A) malaria-positive males, (B) malaria-positive females, (C) malaria-negative males and (D) malaria-negative females. Figure S4. Secondary structure analysis of G6PD variants by circular dichroism. Table S3. Melting temperature (Tm) values of recombinant G6PD proteins by thermal shift assay. Mutations were ranked in order of stability, from most stable to least stable. Table S4. Thermal inactivation of G6PD variants as reported by T1/2. Mutations were ranked in order of stability, from most stable to least stable. Table S5. Stability of G6PD variants in the presence of Gdn-HCl as reported by C1/2. Mutations were ranked in order of stability, from most stable to least stable. Table S6. Susceptibility of G6PD variants to trypsin digestion. Mutations were ranked in order of stability, from most stable to least stable. Table S7. Structural characteristics of the dimer and tetramer interfaces (t = 100 ns). Table S8. Average values of the trajectory analyses performed on the WT and variants. Figure S5. Ligand binding pocket occupancy heatmap indicating the presence (orange) and absence (turquoise) of hydrogen bonds (t = 100 ns). Figure S6. Superimposition and structural deviations of the simulated variants against the WT (red) at the mutation site, dimer and tetramer interfaces (t = 100 ns). (A) Gaohe, (B) Valladolid, (C) Canton, (D) Viangchan, (E) Gond, (F) Gaohe + Viangchan, (G) Valladolid + Viangchan, and (H) Canton + Viangchan