Photograph of training plate without end-titres for training purposes.

<p>Serum dilution is given with (serum plus antigen dilution) in <i>parentheses</i>. A, <1∶ 100; B, <1∶100; C, >1∶102,400; D, 1∶51,200; E, 1∶51,200; F, 1∶12,800; G, 1∶800; H, >1∶102,400 (Serum dilution).</p

Clinical accuracy of DAT test in proficiency panel pre and post refresher training; comparison of two cut-off titres (1∶1,600 and 1∶3,200 serum dilution).

<p>Each reading at every laboratory (n = 10) is treated as a separate sample in this analysis. There are 151 total readings of the DAT as all but one centre tested the same sera; in the final centre one serum sample was replaced with another and also tested by ITM, hence 151 readings.</p><p>TP = True positive, FN = false Negative, FP = False Positive, TN = True Negative, CI = Confidence Intervals.</p

Recommendations from VL-LN for DAT assay.

<p>Recommendations from VL-LN for DAT assay.</p

Set-up of plate.

<p>Serum dilution is given with serum (plus antigen) dilution in parentheses – it is essential to specify the type of dilution used when reporting DAT results.</p

Evaluation centres.

<p>Evaluation centres.</p

Photograph of training plate with end-titres defined by vertical red line.

<p>Where arrows show (row F &H) the end titre can not be calculated without further diluting the serum sample over two plates. Serum dilution is given with (serum plus antigen dilution) in <i>parentheses</i>.</p

A breakdown explaining the equivalent number of trypanosomes at different volumes used in the study.

<p>A breakdown explaining the equivalent number of trypanosomes at different volumes used in the study.</p

Details of five alternative field-friendly DNA extraction methods.

<p>Details of five alternative field-friendly DNA extraction methods.</p

Persistence of <i>T</i>. <i>b</i>. <i>brucei</i> DNA in <i>G</i>. <i>m</i>. <i>morsitans</i>.

<p>Persistence of <i>T</i>. <i>b</i>. <i>brucei</i> DNA in <i>G</i>. <i>m</i>. <i>morsitans</i>.</p

Limit of detection results for LAMP and TBR primers, a total of six flies were used for each dilution gradient for both assays.

<p>Limit of detection results for LAMP and TBR primers, a total of six flies were used for each dilution gradient for both assays.</p