7 research outputs found
ATRX binds to atypical chromatin domains at the 3′ exons of zinc finger genes to preserve H3K9me3 enrichment
<p>ATRX is a SWI/SNF chromatin remodeler proposed to govern genomic stability through the regulation of repetitive sequences, such as rDNA, retrotransposons, and pericentromeric and telomeric repeats. However, few direct ATRX target genes have been identified and high-throughput genomic approaches are currently lacking for ATRX. Here we present a comprehensive ChIP-sequencing study of ATRX in multiple human cell lines, in which we identify the 3′ exons of zinc finger genes (ZNFs) as a new class of ATRX targets. These 3′ exonic regions encode the zinc finger motifs, which can range from 1–40 copies per ZNF gene and share large stretches of sequence similarity. These regions often contain an atypical chromatin signature: they are transcriptionally active, contain high levels of H3K36me3, and are paradoxically enriched in H3K9me3. We find that these ZNF 3′ exons are co-occupied by SETDB1, TRIM28, and ZNF274, which form a complex with ATRX. CRISPR/Cas9-mediated loss-of-function studies demonstrate (i) a reduction of H3K9me3 at the ZNF 3′ exons in the absence of ATRX and ZNF274 and, (ii) H3K9me3 levels at atypical chromatin regions are particularly sensitive to ATRX loss compared to other H3K9me3-occupied regions. As a consequence of ATRX or ZNF274 depletion, cells with reduced levels of H3K9me3 show increased levels of DNA damage, suggesting that ATRX binds to the 3′ exons of ZNFs to maintain their genomic stability through preservation of H3K9me3.</p
Characterization of ERK signaling and H3 post-translational modifications in TICs.
<p>(<b>A</b>) Detection of K27me3, K4me3, EZH2 and P-ERK1/2 by IF in CFSE-labeled HEp3 cells grown <i>in vivo</i> for 4 days. Representative images, LRCs stained for P-ERK (confocal scanning image – scale bar = 60 µm), K27me3, K4me3 and EZH2 (all standard fluorescence microscopy images – scale bar = 80 µm- scale bar inset = 60 µm). Contour of LRCs is delineated with a dashed green line. Arrows = LRCs, arrows with circle = NLRCs. Quantification of marker-positive or negative in LRCS and NLRCs is shown in the graphs on the right. Y axes show the percentage of cells per tumor. Graphs show mean ± SD of three independent tumors. (<b>B</b>) Detection of CD49f, K27me3 and K4me3 in HEp3 cells grown <i>in vivo</i> 6 days. Representative images of CD49f, K27me3 and K4me3, standard fluorescence microscopy images – scale bar = 80 µm- Contour of CD49f<sup>high</sup> cells is delineated with a dashed green line. Arrows = CD49f<sup>high</sup>, arrows with circle = CD49f<sup>low</sup>. Quantification of marker-positive or negative in CD49f<sup>high</sup> and CD49f<sup>low</sup> cells is shown on the graphs on the right. Y axes show the percentage of cells per tumor. Graphs show mean ± SD of three independent tumors. (<b>C</b>) Detection of K27me3 and CD49f in sections from human oral primary tumors (PT68880). Scale bar = 80 µm. CD49f positive cells are delineated with a dashed red line. Y axes show the percentage of cells per section. p-values estimated using Mann-Whitney non-parametric test.</p
CD49f<sup>high</sup> Cells Are A Slow Cycling Population in T-HEp3 Tumors.
<p>(<b>A</b>) Upper panel, strategy to use CFSE (20 µM) to identify LRCs in HEp3 tumors <i>in vivo</i>. CFSE diffuses into the cells and is esterified onto proteins. Cell division dilutes the label and the slow-cycling or growth arrested cells will retain the CFSE (label retaining cells - LRCs). Lower photograph - cytospin from a CFSE-labeled 4 day T-HEp3 tumor grown on the CAM. The LRCs (green cells, arrows) can be easily identified. Scale bar: 80 µm and 40 µm inset scale bar. (<b>B</b>) FACS detection and quantification of the CFSE population after <i>in vivo</i> growth. Upper panel - dot-plot graph of unstained (UN) T-HEp3 tumors; middle panel - CFSE labeled tumors after 7 days (d7) <i>in vivo</i>. Lower panel - percentage of LRCs determined by fluorescence microscopy in HEp3 (T) tumors grown on CAMs or nude mice d4–d12 after injection. Top numbers = mean percent of LRCs per tumor. (<b>C</b>) Detection of CD49f in LRCs and NLRCs in d6 CAM tumors by IF. LRCs (green) are positive for CD49f (red), arrows. Inset, merged image of CD49f and CFSE signals. CFSE signal bathes the whole cell but primarily the cytosol. CD49f signal in permeabilized HEp3 cells excluded completely the nucleus (Blue, DAPI). Scale bar: 60 µm and 40 µm inset scale bar. Lower panel graphs - quantification of CD49f<sup>high</sup> cells in both LRC and NLRC populations. (<b>D</b>) Quantification of surface CD49f<sup>high</sup> cells in both LRC and NLRC populations by FACS. p-values estimated using Mann-Whitney non-parametric test. (<b>E–F</b>) FACS quantification of CD49f expression in LRCs and NLRCs in SQ20b (<b>E</b>) and FaDu (<b>F</b>) tumors grown in nude mice. Statistical significance was estimated using Mann-Whitney non-parametric test and 95% confidence interval.</p
ALDH<sup>high</sup>, CD49f<sup>high</sup>, LRCs proliferative capacity <i>in vivo</i>.
<p>(<b>A</b>) Sorting strategy for HEp3 cells with high or low levels of ALDH, CD49f, or both. (<b>B</b>) Sorting strategy for HEp3 tumors labeled with CFSE. (<b>C–E</b>) Quantification of tumor growth after 1 week <i>in vivo</i> on CAM. Dots represent the number of tumor cells per nodule. CD49f<sup>high</sup> vs. CD49f<sup>low</sup> cells (<b>C</b>), LRC vs. NLRCs (<b>D</b>), and (<b>E</b>) ALDH<sup>high</sup>/CD49f<sup>high</sup> vs. ALDH<sup>low</sup>/CD49f<sup>low</sup>. (<b>F</b>) Quantification of tumor growth after 24 h <i>in vivo</i> on CAM. Dots represent the number of tumor cells per nodule. (<b>G</b>) Representative images of tumor nodules produced by ALDH<sup>high</sup>/CD49f<sup>high</sup> and ALDH<sup>low</sup>/CD49f<sup>low</sup> (left panel) or LRCs and NLRCs (right panel). T = tumor, H = host tissue (CAM), I = inoculation site. Scale bar: 8 mm. p-values estimated using one-way ANOVA followed by the Bonferroni correction with two-tailed <i>P</i> values<0.05 considered significant.</p
CD49f<sup>low</sup>/NLRCs can regain their tumorigenic capacity and restore CD49f and ALDH1A1 expression.
<p>(<b>A</b>) Tumor latency (left panel) and tumor take (Right panel) of HEp3 LRCs and NLRCs that were injected in nude mice (10<sup>3</sup> cells/mice). Graphs show mean ± SD. The numbers represent the number of animals per group. (<b>B</b>) Quantification of tumor growth by CD49f<sup>high</sup>and CD49f<sup>low</sup> HEp3 cells upon serial transplantation on CAMs (1–3 weeks). (<b>C</b>) Tumor latency (left panel) and tumor take (Right panel) of SQ20b LRCs and NLRCs that were injected in NSG mice with matrigel (10<sup>3</sup> cells/mice). Graphs show mean ± SD. The numbers represent the number of animals per group. (<b>D</b>) Tumor latency (left) and tumor take (right) of the progeny of HEp3 LRCs and NLRCs after expansion in culture and injection in nude mice. (<b>E–F</b>) FACS analysis of surface CD49f (<b>E</b>) and ALDH1A1 (<b>F</b>) expression in the <i>in vitro</i> expanded progeny of LRCs and NLRCs (<i>in vitro</i> progeny) (Left part of the graph) and in tumors from the progeny of the LRCs and NLRCs expanded in culture (Tumor progeny)( Right part of the graph). (<b>G</b>) Quantification of HEp3 tumor growth after inhibition of CD49f using siRNA (Lower graph). Inhibition of CD49f expression was measured by FACs (upper left panel) and qPCR (upper right panel) (siC = SiRNA scrambled; siCD49f = siRNA CD49f). (<b>H</b>) Quantification of HEp3 tumor growth after treatment with a CD49f blocking antibody or an isotype matched IgG. p-values estimated using Mann-Whitney non-parametric test.</p
Primary tumors and metastasis from HNSCC patients contain a subpopulation of CD49f<sup>high</sup> cells.
<p>(<b>A–D</b>) Representative CD49f staining in primary tumors (A and C) and lymph node metastasis (B and D) from two different patients. CD49f<sup>high</sup> tumor cells are marked with arrows. Note the strong cytosolic and membrane pattern of the signal (arrowhead in the, inset) and the overall stronger signal and increased frequency for CD49f in the metastatic lesions (B and D). Scale bar: 80 µm and 40 µm inset scale bar. (<b>E–F</b>) Immunofluorescence detection of CD49f in sections from human oral primary tumors (E) and metastasis (F) (PT68880). Scale bar: 60 µm. (<b>G</b>) Quantification of CD49f<sup>high</sup> cells in sections from primary tumors and lymph node metastasis. Columns represent mean of 5 different sections per sample and a minimum of 500 cells was scored per sample. p-values estimated using one-way ANOVA followed by the Bonferroni correction with two-tailed <i>P</i> values<0.05 considered significant.</p
Characterization of H3 post-translational modifications upon knockdown of CD49f.
<p>(<b>A</b>) Immunoblot of phospho- and total-ERK1/2, H3K27me3, H3K4me3, H3K9me3 and EZH2 expression in LRCs and NLRCs sorted and expanded <i>in vitro</i>. Total ERK1/2 and Amido black were used as loading controls. (<b>B</b>) Inhibition of CD49f in HEp3 cells grown <i>in vivo</i> 6 days. Left panels: representative images of CD49f in HEp3 cells transfected with a scrambled siRNA (siRNA neg) or with a siRNA CD49f. The cells were permeabilized, thus CD49f signal bathes the whole cell but primarily the cytosol (scale bar = 60 µm). Insets, overlay of CD49f and DAPI, showing the CD49f characteristic membrane and cytosolic staining (Scale bar = 40 µM). Upper right graph- quantification of CD49f mean fluorescence intensity (MFI). Lower right graph,-quantification of CD49f expression by qPCR. (<b>C</b>). Representative images of K4me3 and K27me3 in HEp3 cells transfected with a scrambled siRNA (siRNA neg) or with a siRNA CD49f. Left panels- standard fluorescence microscopy images – scale bar = 80 µm. Quantification of K27me3<sup>high</sup> and K4me3<sup>high</sup> percentage of cells and mean fluorescence intensity is shown on the graphs on the right. Graphs show mean ± SD of three independent tumors. p-values estimated using Mann-Whitney non-parametric test. (<b>D</b>) Scheme depicting the hypothetical behavior of different populations in HNSCC tumors. Marker high (CD49f<sup>+</sup>) and marker low (CD49f<sup>−</sup>) populations within HNSCC tumors can be defined by their corresponding programs characterized by the markers indicated in the dialogue boxes. CD49f+ can self-renew (path 1) or after transiting through an intermediary state (CD49f<sup>+/−</sup>) (Path 2) fully reprogram into CD49f<sup>−</sup> (Path2+3). The intermediary state (CD49f<sup>+/−</sup>) is defined by default by the markers in the dialogue box. The MTP model proposed that CD49f<sup>−</sup> cells can also transit through the intermediary state (Path 4) to then fully reprogram into CD49f<sup>+</sup> (Paths 4+5). Our model also considers that the different paths are stochastic and tumor cells might transition back and forth between the different states. A remaining open question is what controls the dynamic plasticity that drives the alternation between these states (question mark).</p