Arterial structure in mesenteric arteries submitted to high blood flow.

<p>Arterial media thickness (A), media cross-section area (B) and media to lumen ratio (C) were measured in mesenteric arteries submitted to a chronic increase in blood flow (high flow: HF) and in control arteries submitted to normal flow (NF). Arteries were isolated from ovariectomized rats treated with resveratrol 5.0 (R5.0 or Resv. 5.0, n = 9 rats) or 37.5mg/kg (R37.5 or Resv. 37.5, n = 9 rats) or with the vehicle (Veh., n = 10 rats). Mean ± sem is represented. *P<0.05, HF versus NF arteries. ^#P<0.05, R37.5 versus vehicle.</p

Phenylephrine-mediated contraction in mesenteric arteries submitted to high blood flow.

<p>Phenylephrine-mediated contraction was measured in mesenteric arteries submitted to a chronic increase in blood flow (high flow: HF, right panel) and in control arteries submitted to normal flow (NF, left panel). Arteries were isolated from ovariectomized rats treated with resveratrol 5.0 (R5.0 or Resv. 5.0, n = 9 rats) or 37.5mg/kg (R37.5 or Resv. 37.5, n = 9 rats) or with the vehicle (n = 10 rats). Mean ± sem is represented. *P<0.05, HF versus NF arteries. ^#P<0.05, R37.5 versus vehicle.</p

Mitochondrial proteins expression level.

<p>The expression level of of Cytochrome-C Oxidase IV (COX IV, A), sirtuin-1 (B), pGC1alpha (C), and Cytochrome C (D) was determined using Western-blot analysis in mesenteric arteries (left panel). The ratio of the expression level in HF to the expression level in NF arteries is shown in the right panel. Arteries were isolated from ovariectomized rats treated with resveratrol 5.0 (Resv. 5.0, n = 9 rats) or 37.5mg/kg (Resv. 37.5, n = 9 rats) or with the vehicle (n = 10 rats). Mean ± sem is represented (n = 12 rats per group). Blots are shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0146148#pone.0146148.s003" target="_blank">S3A–S3D Fig</a>. *P<0.05, effect of the treatment: Resv. 5.0 or Resv. 37.5 versus vehicle.</p

Expression level of eNOS (A), p67 phox (B), Gp91 phox (C) and ERK1/2 activation (D, ratio of phospho-ERK1/2 to ERK1/2) expression level.

<p>The ratio of protein expression in HF to the expression level in NF arteries is shown in the right panel. Arteries were isolated from ovariectomized rats treated with resveratrol 5.0 (Resv. 5.0, n = 9 rats) or 37.5mg/kg (Resv. 37.5, n = 9 rats) or with the vehicle (n = 10 rats). Mean ± sem is represented. Blots are shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0146148#pone.0146148.s003" target="_blank">S3A–S3D Fig</a>. *P<0.05, effect of the treatment: Resv. 5.0 or Resv. 37.5 versus vehicle.</p

Arterial diameter in mesenteric arteries submitted to high blood flow.

<p>Luminal diameter was measured in mesenteric arteries submitted to a chronic increase in blood flow (high flow: HF) and in control arteries submitted to normal flow (NF). Arteries were isolated from ovariectomized rats treated with resveratrol 5.0 (B, n = 9 rats) or 37.5mg/kg (C, n = 9 rats) or with the vehicle (A, n = 10 rats). In a separate series of experiments, ERalpha-/- mice were ovariectomized and treated with the solvent or with resveratrol 5mg/kg (D, n = 4 mice per group). Mean ± sem is represented. *P<0.05, HF versus NF arteries.</p