Z score values of samples calculated by three different previously published methods.

<p><b>□</b> –trisomic samples, <b>○</b> –euploid samples, horizontal line—mean z score value of trisomic samples. Dotted lines represent the standard limit for identification of a trisomic sample (z score = 3). A—Ion Torrent PGM analyzed samples, B—MiSeq analyzed samples.</p

Z score values calculated from reads without size selection (all), after <i>in silico</i> size selection (IS) and after physical size selection (P).

<p>Horizontal lines represent mean z score value calculated from 3, 2 and 1 million raw reads (3m, 2m, 1m). Dotted lines represent the standard limit for identification of a trisomic sample (z score = 3). A—Ion Torrent PGM analyzed samples, B—MiSeq analyzed samples.</p

Z score values of trisomic and euploid samples before and after <i>in silico</i> size selection of sequencing reads.

<p><b>□</b> –trisomic samples, <b>○</b> –euploid samples, horizontal line—mean z score value). Dotted lines represent the standard limit for identification of a trisomic sample (z score = 3). A—Ion Torrent PGM analyzed samples, B—MiSeq analyzed samples.</p

Determination of optimal <i>in silico</i> size selection limit for sequencing reads to be used in z score calculation.

<p>Dotted lines represent the standard limit for identification of a trisomic sample (z score = 3). A—Ion Torrent PGM analyzed samples, B—MiSeq analyzed samples.</p