Raw data in excel format and original gel images.

(XLSX)</p

Agarose gel electrophoresis for Bruce–ladder PCR products amplified from cultures of tissues from slaughtered cattle.

Lanes M: Gene Ruler 100 bp (ThermoFischer Scientific, Johannesburg, South Africa); lanes 1–5: B. abortus; lanes 6–8: B. melitensis; lane 9: positive control, B. suis ZW45, lane 10: positive control, B. melitensis rev 1, lane 11: B. abortus (REF 544), lane 12: positive control, B. abortus S 19, lane 13: negative control with sterile water.</p

Univariable associations between animal characteristics and ITS PCR assay of <i>Brucella</i> spp. isolates from cultures of tissues of slaughtered cattle in Rwanda.

Univariable associations between animal characteristics and ITS PCR assay of Brucella spp. isolates from cultures of tissues of slaughtered cattle in Rwanda.</p

Sequences of oligonucleotide primers used for the distinction of <i>Brucella</i> species isolated from slaughtered cattle in Rwanda.

Sequences of oligonucleotide primers used for the distinction of Brucella species isolated from slaughtered cattle in Rwanda.</p

Agarose gel electrophoresis of the 16-23S interspacer region (ITS) PCR products amplified from cultures of tissues from slaughtered cattle.

Lanes M: DNA Gene Ruler 100bp plus (Thermo Fischer Scientific, Johannesburg, South Africa), lanes 1–7: amplification of a 214 bp sequence of the genus Brucella spp., lane 8: negative control containing sterile water, lane 9: positive control with B. abortus RF544.</p

A map of Rwanda with provinces and districts with red stars showing the locations of abattoirs visited in this study [22].

A map of Rwanda with provinces and districts with red stars showing the locations of abattoirs visited in this study [22].</p

Agarose gel electrophoresis for AMOS PCR products amplified from cultures of tissues from slaughtered cattle.

Lanes M: Gene Ruler 100 pb plus (ThermoFischer Scientific, Johannesburg, South Africa), lanes 1–4: Brucella abortus (496 bp), lanes 5–7: B. melitensis (731 bp), Lanes 9–10: mixed B. melitensis and B. abortus, lane 11: negative control containing sterile water, lane 12: positive control, B. abortus RF544, lane 13: positive control, B. melitensis rev 1.</p

Serological, bacterial culture, and PCR results of samples collected from slaughtered cattle in Rwanda.

Serological, bacterial culture, and PCR results of samples collected from slaughtered cattle in Rwanda.</p