37 research outputs found

    USES OF THE PSEUDOMONAS SYRINGAE EFFECTOR PROTEIN HOPU1 RELATED TO ITSABILITY TO ADP-RBOSYLATE EUKARYOTC RNA BINDING PROTENS

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    The bacterial plant pathogen Pseudomonas Syringae injects effector proteins into host cells via a type III protein secre tion system to cause disease. The invention relates to the discovery that the type III effector HopU1 is a mono-ADP ribosyltransferase (ADP-RT) and suppresses plant innate immunity. The HopU1 substrates in Arabidopsis thaliana extracts were RNA-binding proteins that possess RNA recognition motifs (RRMs). A. thaliana knock-out lines defective in the glycine-rich RNA-binding protein AtGRP7, a HopU1 substrate, were more susceptible than wild type plants to P syringae. The ADP-ribosylation of AtGRP7 by HopU1 required two arginines within the RRM. The inven tion provides novel methods for the modulation of the innate immune response of a plant to a biotic stress, including methods for enhancing or suppressing the innate immune response of the plant

    TRANSGENIC SOYBEAN PLANTS EXPRESSING ASOYBEAN HOMOLOG OF GLYCINE-RICH PROTEIN 7 (GRP7) AND EXHIBITING IMPROVED INNATE IMMUNITY

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    This disclosure provides for transgenic Soybean plants expressing a soybean homolog of glycine-rich protein 7 (GRP7) and exhibiting improved innate immunity and meth ods of making Such plants

    TRANSGENIC SOYBEAN PLANTS EXPRESSING ASOYBEAN HOMOLOG OF GLYCINE-RICH PROTEIN 7 (GRP7) AND EXHIBITING IMPROVED INNATE IMMUNITY

    Get PDF
    This disclosure provides for transgenic Soybean plants expressing a soybean homolog of glycine-rich protein 7 (GRP7) and exhibiting improved innate immunity and meth ods of making Such plants

    USES OF THE PSEUDOMONAS SYRINGAE EFFECTOR PROTEIN HOPU1 RELATED TO ITSABILITY TO ADP-RBOSYLATE EUKARYOTC RNA BINDING PROTENS

    Get PDF
    The bacterial plant pathogen Pseudomonas Syringae injects effector proteins into host cells via a type III protein secre tion system to cause disease. The invention relates to the discovery that the type III effector HopU1 is a mono-ADP ribosyltransferase (ADP-RT) and suppresses plant innate immunity. The HopU1 substrates in Arabidopsis thaliana extracts were RNA-binding proteins that possess RNA recognition motifs (RRMs). A. thaliana knock-out lines defective in the glycine-rich RNA-binding protein AtGRP7, a HopU1 substrate, were more susceptible than wild type plants to P syringae. The ADP-ribosylation of AtGRP7 by HopU1 required two arginines within the RRM. The inven tion provides novel methods for the modulation of the innate immune response of a plant to a biotic stress, including methods for enhancing or suppressing the innate immune response of the plant

    Characterization and Solubilization of the Alternative Oxidase of Sauromatum guttatum

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