miR-146b-5p inhibition prevents the anti-inflammatory action of globular adiponectin.

<p>(<b>A</b>) TNFα RNA levels and soluble TNFα protein levels, (<b>B</b>) IRAK3 RNA and protein levels, (<b>C</b>) m- and iROS production, (<b>D</b>) IRS1 RNA and protein levels in THP-1 cells exposed to 10 µg/ml globular adiponectin with (n = 4) or without (n = 6) inhibition of miR-146b-5p. Data shown are means ± SEM. ^*<i>P</i><0.05, ^**<i>P</i><0.01 and ^***<i>P</i><0.001 compared with THP-1 cells exposed to 10 µg/ml globular adiponectin without miR-146b-5p inhibition. Abbreviations: iROS, intracellular ROS; mROS, mitochondrial ROS; ROS, reactive oxygen species.</p

Gene expressions in monocytes of obese subjects.

<p>Data shown are means ± SEM.</p>***<p><i>P</i><0.001 obese compared with lean controls.</p

miR-146b-5p expression is decreased in monocytes of obese subjects and is regulated by globular adiponectin.

<p>(<b>A</b>) miR-146b-5p expression levels in isolated monocytes from 14 lean controls and 21 obese subjects as determined by qRT-PCR analysis. Data are expressed as means. ^**<i>P</i><0.01 obese compared with lean controls. miR-146b-5p expression levels determined by qRT-PCR in THP-1 cells exposed to (<b>B</b>) 1 or 10 µg/ml globular adiponectin for 24 h (n = 6), and (<b>C</b>) 5.5 mM D-glucose and 9.5 mM D-mannitol (osmotic control) or 15 mM D-glucose for 24 h (n = 6). Data shown are means ± SEM. ^*<i>P</i><0.05 compared with THP-1 cells exposed to 10 µg/ml globular adiponectin.</p