167 research outputs found
Fatty acid composition and sensory acceptability of dry cured ham influenced by linseed oil, fish oil or microalgae included in the pig feed
Performance, carcass and ham quality in crossbreds combinations of three sow lines and two Belgian Piétrain lines
Performance, carcass and meat quality differences between gilts and immunocastrates in diverse pig crossbreeds
Straightforward and sensitive RT-qPCR based gene expression analysis of FFPE samples
Fragmented RNA from formalin-fixed paraffin-embedded (FFPE) tissue is a known obstacle to gene expression analysis. In this study, the impact of RNA integrity, gene-specific reverse transcription and targeted cDNA preamplification was quantified in terms of reverse transcription polymerase chain reaction (RT-qPCR) sensitivity by measuring 48 protein coding genes on eight duplicate cultured cancer cell pellet FFPE samples and twenty cancer tissue FFPE samples. More intact RNA modestly increased gene detection sensitivity by 1.6 fold (earlier detection by 0.7 PCR cycles, 95% CI = 0.593-0.850). Application of gene-specific priming instead of whole transcriptome priming during reverse transcription further improved RT-qPCR sensitivity by a considerable 4.0 fold increase (earlier detection by 2.0 PCR cycles, 95% CI = 1.73-2.32). Targeted cDNA preamplification resulted in the strongest increase of RT-qPCR sensitivity and enabled earlier detection by an average of 172.4 fold (7.43 PCR cycles, 95% CI = 6.83-7.05). We conclude that gene-specific reverse transcription and targeted cDNA preamplification are adequate methods for accurate and sensitive RT-qPCR based gene expression analysis of FFPE material. The presented methods do not involve expensive or complex procedures and can be easily implemented in any routine RT-qPCR practice
Evaluation des performances, la qualité de carcasse et de viande de porcs femelles et mâles immuno-castrés issus de différents verrats terminaux
In-depth investigation of diet-related DNA adduct formation via DNA adductomics : to eat or not to eat red meat!?
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