Cell-free DNA level in follicular fluid pools according to the patients’ ovarian reserve status, ovarian reserve parameters and infertility length.

<p><b>A</b>, Follicular fluid cfDNA content in patients with normal ovarian reserve versus patients with ovarian reserve disorders (ovarian insufficiency and polycystic ovary syndrome); *p = 0.03. <b>B</b>, Follicular fluid cfDNA content according to the ovarian reserve parameters; left panel: AFC (<10 versus ≥ 10, *p = 0.04); right panel: AMH (≤ 1 versus > 1 ng/ml, *p = 0.06). <b>C</b>, Follicular fluid cfDNA levels according to the infertility length (1 versus ≥ 5 years, *p = 0.049).</p

ROC curve to evaluate the predictive value of follicular fluid cfDNA level for clinical pregnancy outcome in a multivariate model (including the rank of IVF/ICSI attempts and the number of embryos): area under the curve = 0.73 [0.66–0.87], sensitivity = 60%, specificity = 88%.

<p>ROC curve to evaluate the predictive value of follicular fluid cfDNA level for clinical pregnancy outcome in a multivariate model (including the rank of IVF/ICSI attempts and the number of embryos): area under the curve = 0.73 [0.66–0.87], sensitivity = 60%, specificity = 88%.</p

CfDNA level in follicular fluid pools according to the ovarian stimulation protocol and ovarian response.

<p><b>A</b>, Follicular fluid cfDNA content according to the length of ovarian stimulation (≤ 10 versus > 10 days, *p = 0.008). <b>B</b>, Follicular fluid cfDNA content according to the total dose of gonadotropins (<3000 versus ≥3000 IU/l, *p = 0.01). <b>C</b>, Follicular fluid cfDNA content according to the number of retrieved oocytes (≤ 6 versus > 6 oocytes, *p = 0.045).</p

CfDNA level in follicular fluid pools according to the embryo outcome at day 2 and 3.

<p><b>A</b>, Follicular Fluid cfDNA content according to the total number of embryos at day 2 (≤ 2 versus > 2, *p = 0.03). <b>B</b>, Follicular Fluid cfDNA content according to, left panel: the number of top quality (grade 1–2) embryos per patient (0 versus ≥ 1, *p = 0.002) at day 2, right panel: ratio between number of top quality embryos and total number of embryos (< 0.2 versus ≥ 0.2, *p = 0.04) at day 2. <b>C</b>, Follicular Fluid cfDNA content according to, left panel: number of top quality (grade 1–2) embryos per patient (0 versus ≥ 1, *p = 0.006) at day 3, right panel: ratio between number of top quality embryo and total number of embryos (< 0.2 versus ≥ 0.2, *p = 0.02) at day 3. <b>D</b>, Follicular Fluid cfDNA content according to, left panel: fragmentation rate at day 3 (<20% versus ≥ 20%, p = 0.18) and right panel: ratio between blastomere number and total embryo number at day 3 (<6 versus 6–8, *p = 0.02).</p

Additional file 2: Table S2. of Apolipoprotein B is regulated by gonadotropins and constitutes a predictive biomarker of IVF outcomes

Description of patients' characteristics according to APOB concentrations divided into quartiles. Q1, quartile 1; Q2, quartile 2; Q3, quartile 3; Q4, quartile 4; SD, Standard Deviation; CI, Confidence Intervals, BMI, Body Mass Index; IVF, In Vitro Fertilization; ICSI, Intracytoplasmic Sperm Injection. (DOCX 15 kb