1H, 13C and 17O isotropic and anisotropic hyperfine coupling prediction for the tyrosyl radical using hybrid density functional methods

AbstractHybrid density functional calculations are used to directly calculate the principal hyperfine tensor values for 1H, 13C and 17O in two models of the tyrosyl radical, p-methylphenoxyl and p-ethylphenoxyl. Both hydrogen bonded and non-hydrogen bonded phenoxyl radicals are studied. A comparison is made between calculated values and those obtained from experimental EPR and ENDOR studies. Outstanding agreement between experiment and theory is observed

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead