Metabolism and Solar Radiation in Dark and White Herons Nesting in Hot Climates

Metabolism, body temperature (Tb), and feather reflectance was measured for dark Louisiana herons (LOU), little blue herons (LB), snowy egrets (SE), and cattle egrets (CE); only LOU nest preferentiallyin shade. The reflectance of LOU and adult LB was 13%-15%; it was 80%-82% for SE, CE, and immature (white) LB. Tb for all four species approximated 40 C; hypothermy was never observed. Basal metabolism (HJb) was 107% of the expected level for LOU, 85% for SE and CE, and 66% for LB. Minimal thermal conductance (C) was calculated as approximately 131% of the expected value for LOU, 115% for SE and CE, and 110% for LB. Metabolism and solar radiation may combine to produce heat stress in hot climates, particularly in dark birds; reduced Hb allows LB and other dark birds to nest in exposed sites. High values for C suggest a heat dissipation function; the relatively lower values are found in species with relatively lower Hb\u27S, thus preventing or reducing increases in T1, the lower limit of thermoneutrality. White plumage is thermally advantageous in tropical/subtropical birds nesting in open habitat, but dark coloration is not; other evolutionary explanations must be sought to explain the occurrence of dark birds in hot climates

Daily energy expenditure of Florida Scrub-Jays (Aphelocoma coerulescens) in suburban and wildland landscapes

Urbanization alters ecological systems, disturbance regimes, food webs, and a variety of other processes that can influence local flora and fauna. In birds, most studies have focused on behavioral or demographic responses to altered conditions; however, the physiological mechanisms associated with these responses have been understudied. We have previously documented shifts in foraging behavior because of the availability of human-provided food; we sought to see if this was driven by or associated with a change in field metabolic rates. We measured field metabolic rates (FMR), a measure of daily energy expenditure (DEE), of Florida Scrub-Jays (Aphelocoma coerulescens) in 2 habitats using doubly labeled water (DLW). One population of jays lived in wildland habitat, the other in a suburban area where the habitat was badly degraded. During the breeding season FMR of suburban males exceeded that of wildland males by over 100%. Female FMR did not differ between sites but increased following incubation. In the wildlands, FMRs of females were barely lower than those of males, but in the suburban landscape, female FMRs were much lower than in males. For both sexes in the wildlands, FMRs were about 3.53 basal rates of metabolism (BMR) during breeding; in the suburbs female FMR exceeded BMR by 43 but did not differ from that of wildland females. Suburban male FMRs exceeded BMR by 7.13. During the nonbreeding seasons of fall and winter, FMR did not differ between habitats, ranging from 1.73 to 2.13 BMR, thus it is the breeding season that separated wildland and suburban jays energetically. In both habitats, a low water economy index (mL/kJ) and low water fluxes indicated that these jays are well adapted to their xeric habitat, but their FMRs distinguished them from desert birds. The very high energetic cost associated with breeding for suburban males may help explain why in the metapopulation of Florida Scrub-Jays, suburban jay populations are sinks. This study underscores the value of physiology in the conservation of listed species

Total Body Water and Body Composition in Phalaropes and Other Birds

Contrary to some reviews, total body water (TBW) may fall below the expected value of about 65% in birds if measured as a percentage of total body mass. However, water constitutes a relatively stable proportion (659%-70%) of body composition when measured as a percentage of lean body mass (LBM). We demonstrate this by using data from two migratory phalaropes (Phalaropus tricolor and Phalaropus lobatus), which exhibit inverse relationships between TBW and body mass and between TBW and fat content. As the phalaropes fatten before migration, lipids increase but not at the expense of water, which also increases with gains in lean tissue. The gain in lean dry mass with body size is also correlated with migration. Because absolute water increases with body mass, TBW is a poor measure of hydration; however, because of the inverse relationship between TBW and fat, TBW can predict body fat accurately if a calibration curve is first generated. We provide equations that predict fat content in both species of phalaropes suggest that water normally be reported not as TBW (a percentage of total body mass) but preferably as lean body water (LBW, a percentage of lean body mass). In the absence of quantitative estimates off at, we suggest that water be communicated in absolute amount, preferably with some estimate of condition (fat)

Temperature Regulation and the Constraints of Climate in the Eared Grebe

Eared Grebes (Podiceps nigricollis) encounter a variety of climatic regimes in their annual cycle. The most dramatic occur while on staging areas in autumn. We investigated the thermoregulatory abilities of the Eared Grebe to determine how they coped with these climate changes during staging. The basal metabolic rate (BMR) was higher than predicted. Mass-specific BMR was 1.212 mL O2/g·h for birds averaging 317 g. The thermoneutral zone is wide and extended from 15°C to 38°C. Minimal thermal conductance and average body temperature were not unusual. We conclude that Eared Grebes live most of the year under thermoneutral conditions and that food availability, not temperature extremes, determines the timing of their winter migration

Incubation Energetics of the Laysan Albatross

The energy expenditure of incubating and foraging Laysan Albatross (Diomedea immutabilis, mean body weight 3.07 kg) was estimated by means of the doubly-la- belled water technique. During incubation, the energy expenditure was similar to that of resting birds that were not incubating an egg. The energy expenditure of foraging albatross (2072 kJ/day) was 2.6 times that of resting birds. It was concluded that the energy expenditure of the tropical Laysan Albatross was not less than that of species foraging over cold, high-latitude oceans. An energy budget compiled for an incubating pair of albatross revealed that the energy expenditure of the female was greater than that of the male bird, during the incubation perio

Measuring inorganic phosphate and intracellular pH in the healthy and hypertrophic cardiomyopathy hearts by in vivo 7T 31P-cardiovascular magnetic resonance spectroscopy.

BACKGROUND: Cardiovascular phosphorus MR spectroscopy (31P-CMRS) is a powerful tool for probing energetics in the human heart, through quantification of phosphocreatine (PCr) to adenosine triphosphate (ATP) ratio. In principle, 31P-CMRS can also measure cardiac intracellular pH (pHi) and the free energy of ATP hydrolysis (ΔGATP). However, these require determination of the inorganic phosphate (Pi) signal frequency and amplitude that are currently not robustly accessible because blood signals often obscure the Pi resonance. Typical cardiac 31P-CMRS protocols use low (e.g. 30°) flip-angles and short repetition time (TR) to maximise signal-to-noise ratio (SNR) within hardware limits. Unfortunately, this causes saturation of Pi with negligible saturation of the flowing blood pool. We aimed to show that an adiabatic 90° excitation, long-TR, 7T 31P-CMRS protocol will reverse this balance, allowing robust cardiac pHi measurements in healthy subjects and patients with hypertrophic cardiomyopathy (HCM). METHODS: The cardiac Pi T1 was first measured by the dual TR technique in seven healthy subjects. Next, ten healthy subjects and three HCM patients were scanned with 7T 31P-MRS using long (6 s) TR protocol and adiabatic excitation. Spectra were fitted for cardiac metabolites including Pi. RESULTS: The measured Pi T1 was 5.0 ± 0.3 s in myocardium and 6.4 ± 0.6 s in skeletal muscle. Myocardial pH was 7.12 ± 0.04 and Pi/PCr ratio was 0.11 ± 0.02. The coefficients of repeatability were 0.052 for pH and 0.027 for Pi/PCr quantification. The pH in HCM patients did not differ (p = 0.508) from volunteers. However, Pi/PCr was higher (0.24 ± 0.09 vs. 0.11 ± 0.02; p = 0.001); Pi/ATP was higher (0.44 ± 0.14 vs. 0.24 ± 0.05; p = 0.002); and PCr/ATP was lower (1.78 ± 0.07 vs. 2.10 ± 0.20; p = 0.020), in HCM patients, which is in agreement with previous reports. CONCLUSION: A 7T 31P-CMRS protocol with adiabatic 90° excitation and long (6 s) TR gives sufficient SNR for Pi and low enough blood signal to permit robust quantification of cardiac Pi and hence pHi. Pi was detectable in every subject scanned for this study, both in healthy subjects and HCM patients. Cardiac pHi was unchanged in HCM patients, but both Pi/PCr and Pi/ATP increased that indicate an energetic impairment in HCM. This work provides a robust technique to quantify cardiac Pi and pHi.This work was funded by a Sir Henry Dale Fellowship from the Wellcome Trust and the Royal Society (grant #098436/Z/12/B to C.T.R.) and by an Erwin Schrödinger Fellowship from the Austrian Science Fund (grant #J4043). Authors also acknowledge the support of the NIHR Oxford Biomedical Research Centre and the Oxford British Heart Foundation Centre of Research Excellence. The support of the Slovak Grant Agency VEGA (grant #2/0001/17) and APVV (grant #15-0029) is also acknowledged

Inhibition of Progenitor Dendritic Cell Maturation by Plasma from Patients with Peripartum Cardiomyopathy: Role in Pregnancy-associated Heart Disease

Dendritic cells (DCs) play dual roles in innate and adaptive immunity based on their functional maturity, and both innate and adaptive immune responses have been implicated in myocardial tissue remodeling associated with cardiomyopathies. Peripartum cardiomyopathy (PPCM) is a rare disorder which affects women within one month antepartum to five months postpartum. A high occurrence of PPCM in central Haiti (1 in 300 live births) provided the unique opportunity to study the relationship of immune activation and DC maturation to the etiology of this disorder. Plasma samples from two groups (n = 12) of age- and parity-matched Haitian women with or without evidence of PPCM were tested for levels of biomarkers of cardiac tissue remodeling and immune activation. Significantly elevated levels of GM-CSF, endothelin-1, proBNP and CRP and decreased levels of TGF- were measured in PPCM subjects relative to controls. Yet despite these findings, in vitro maturation of normal human cord blood derived progenitor dendritic cells (CBDCs) was significantly reduced (p < 0.001) in the presence of plasma from PPCM patients relative to plasma from post-partum control subjects as determined by expression of CD80, CD86, CD83, CCR7, MHC class II and the ability of these matured CBDCs to induce allo-responses in PBMCs. These results represent the first findings linking inhibition of DC maturation to the dysregulation of normal physiologic cardiac tissue remodeling during pregnancy and the pathogenesis of PPCM