Use of mathematical derivatives (time-domain differentiation) on chromatographic data to enhance the detection and quantification of an unknown 'rider' peak

Two samples of an anticancer prodrug, AQ4N, were submitted for HPLC assay and showed an unidentified impurity that eluted as a 'rider' on the tail of the main peak. Mathematical derivatization of the chromatograms offered several advantages over conventional skimmed integration. A combination of the second derivative amplitude and simple linear regression gave a novel method for estimating the true peak area of the impurity peak. All the calculation steps were carried out using a widely available spreadsheet program. (C) 2003 Elsevier B.V. All rights reserved

A Descriptive Study of the Population Dynamics of Adult \u3ci\u3eDiabrotica Virgifera Virgifera\u3c/i\u3e (Coleoptera: Chrysomelidae) in Artificially Infested Corn Fields

The influence of corn plant phenology on the dynamics of adult western corn rootworm, Diabrotica virgifera virgifera, populations was studied during 1988 and 1989 in com fields artificially infested with eggs. Fifty percent of adult emergence from the soil occurred by day 194 in 1988 and day 203 in 1989. In both years, adult emergence was synchronized with corn flowering, eggs were recovered in soil samples approximately four days after reproductive females were first observed in the population, and oviposition was essentially complete about 25 days after it began. The number of reproductive female beetle-days accumulating per m2 was similar in both years. Approximately two times as many eggs were laid in 1988 (1239 eggs 1m2) as in 1989 (590 eggs 1m2). The difference in egg density may have been caused by differences among years in the temporal synchrony of reproductive beetles with flowering corn. Daily survival rates of adults were high while corn was flowering; exhibited a gradual decline during grain filling; and decreased rapidly during the grain drying stage

Genetic Differentiation Among Three Species of \u3ci\u3eParadosa\u3c/i\u3e (Arachnida: Lycosidae)

Allozymic variation in nine protein producing loci was examined in three species of Pardosa using starch gel electrophoresis. Allozyme frequencies showed a high degree of geographic uniformity among conspecific populations. Estimated heterozygosities for the three species ranged from 0.05 to 0.15. Rogers\u27 coefficients of genetic similarity based on allozyme frequencies averaged over conspecific populations ranged from 0.16 to 0.37 fo rthe three species

Functional analysis of the Bunyamwera orthobunyavirus Gc glycoprotein

The virion glycoproteins Gn and Gc of Bunyamwera orthobunyavirus (family Bunyaviridae) are encoded by the M RNA genome segment and have roles in both viral attachment and membrane fusion. To investigate further the structure and function of the Gc protein in viral replication, we generated 12 mutants that contain truncations from the N terminus. The effects of these deletions were analysed with regard to Golgi targeting, low pH-dependent membrane fusion, infectious virus-like particle (VLP) formation and virus infectivity. Our results show that the N-terminal half (453 residues) of the Gc ectodomain (909 residues in total) is dispensable for Golgi trafficking and cell fusion. However, deletions in this region resulted in a significant reduction in VLP formation. Four mutant viruses that contained N-terminal deletions in their Gc proteins were rescued, and found to be attenuated to different degrees in BHK-21 cells. Taken together, our data indicate that the N-terminal half of the Gc ectodomain is dispensable for replication in cell culture, whereas the C-terminal half is required to mediate cell fusion. A model for the domain structure of the Gc ectodomain is proposed