Ultra-sensitive detection of prion protein fibrils by flow cytometry in blood from cattle affected with bovine spongiform encephalopathy-2

<p><b>Copyright information:</b></p><p>Taken from "Ultra-sensitive detection of prion protein fibrils by flow cytometry in blood from cattle affected with bovine spongiform encephalopathy"</p><p>BMC Biotechnology 2005;5():26-26.</p><p>Published online 4 Oct 2005</p><p>PMCID:PMC1266054.</p><p>Copyright © 2005 Trieschmann et al; licensee BioMed Central Ltd.</p>yzed by flow cytometry. The measurements are shown in a Fluorescence 1 (FL1-H) vs. Side-Scatter (SSC) dot-plot. All six BSE-samples (A-F) can be differentiated from the controls (G-J) by a population of events in region R3 (green dots). Panel K: Quantification of measurements shown in panels A-J

Ultra-sensitive detection of prion protein fibrils by flow cytometry in blood from cattle affected with bovine spongiform encephalopathy-0

<p><b>Copyright information:</b></p><p>Taken from "Ultra-sensitive detection of prion protein fibrils by flow cytometry in blood from cattle affected with bovine spongiform encephalopathy"</p><p>BMC Biotechnology 2005;5():26-26.</p><p>Published online 4 Oct 2005</p><p>PMCID:PMC1266054.</p><p>Copyright © 2005 Trieschmann et al; licensee BioMed Central Ltd.</p>0 μl PBS (left panel) or in the same volume of serum (right panel), followed by flow cytometry. The measurements are depicted in a Fluorescence 1 (FL1-H) vs. Fluorescence 2 (FL2-H) dot-plot. The number of counts in the area containing specific signals (R2) is given in the figures. Aggregate formation in serum is strongly inhibited

Ultra-sensitive detection of prion protein fibrils by flow cytometry in blood from cattle affected with bovine spongiform encephalopathy-1

<p><b>Copyright information:</b></p><p>Taken from "Ultra-sensitive detection of prion protein fibrils by flow cytometry in blood from cattle affected with bovine spongiform encephalopathy"</p><p>BMC Biotechnology 2005;5():26-26.</p><p>Published online 4 Oct 2005</p><p>PMCID:PMC1266054.</p><p>Copyright © 2005 Trieschmann et al; licensee BioMed Central Ltd.</p>(panel A) or presence (panel B) of 10nM PrP aggregates. Panel C: quantification of measurements shown in A and B, and of measurements (not shown) with different seed concentrations. The measurements are depicted in a Fluorescence 1 (FL1-H) vs. Side-Scatter (SSC) dot-plot. Aggregate formation (signal in region R1) was strongly enhanced by all seed concentrations tested, from 5 nM to 10nM

Additional file 8: Figure S6. of BMP-SMAD signalling output is highly regionalized in cardiovascular and lymphatic endothelial networks

BRE::gfp transcriptional activity does not co-localise with Ki67 in ECs. (A) Skin biopsy of an E14.5 BRE::gfp mouse with immunodetection of GFP, pH3 and PROX1. The merged stack in the left panel is split up in some single z-stacks to show pH3-positive cells beneath or above vessel structures (arrows). E14.5 (B) and E16.5 (C) skin biopsies with immunodetection of a different proliferation marker (Ki67), GFP and PROX1. Boxed areas are enlarged in the right panels. Ki67-positive LECs (arrows) and blood ECs (arrowheads) can be observed. Scale bars: 75 μm (B–C, left panels); 25 μm (A; B–C, right panels). (PDF 184 kb

Additional file 2: Figure S1. of BMP-SMAD signalling output is highly regionalized in cardiovascular and lymphatic endothelial networks

The BRE::gfp reporter is transcriptionally active in a subdomain of the pSMAD1/5/9-positive endothelial cells. (A–C) Immunodetection of SMAD1/5/9 and GFP in the AVC and OFT at E9.5 (A), the ventricular trabeculae (B) and the cardinal vein (C) at E11.5. DAPI is used to stain nuclei. Specificity of the pSMAD1/5/9 staining was confirmed in control embryos (D) and endothelium-specific Smad1; Smad5 double knockout embryos (Tie2cre+/0; Smad1fl/fl; Smad5fl/fl) (E). Embryos were analyzed at E8.5 to circumvent the embryonic lethality of mutant embryos at E9.5. The pSMAD1/5/9 levels were specifically reduced in endothelium of the EC-specific Smad1; Smad5 double knockouts, while levels remained unchanged in the non-endothelial cells. The myocardium was visualized by an anti-MF20 staining. Arrows indicate pSMAD1/5/9 deficient endothelium (E). At: atrium; AVC: atrioventricular canal; CV: cardinal vein; OFT: outflow tract. Scale bars: 100 μm (A–B;D–E); 50 μm (C). (PDF 2307 kb

Additional file 3: Figure S2. of BMP-SMAD signalling output is highly regionalized in cardiovascular and lymphatic endothelial networks

BRE::gfp localisation patterns in the P10 retina. (A–D) Immunodetection of Endomucin and GFP in the retina at P10. The retina has a multi-layered vasculature that consist of the vascular border (A) and centre (B) of the primary plexus, the perpendicular vessels (C) and outer plexus (D). Single staining for GFP is shown in the lower panels. Scale bars: 75 μm. (PDF 2844 kb