Semi-industrial development of nutritious and healthy seafood dishes from sustainable species

This study aimed to devise innovative, tailor-made, appealing, tasty and semi-industrialized dishes, using sustainable and under-utilized seafood species (bib, common dab, common carp, blue mussel and blue whiting), that can meet the specific nutritional and functional needs of children (8-10-years), pregnant women (20-40-years) and seniors (≥60-years). Hence, contests were organised among cooking schools from 6 European countries and the best recipes/dishes were reformulated, semi-industrially produced and chemically and microbiologically evaluated. The dishes intended for: (i) children and pregnant women had EPA + DHA and I levels that reached the target quantities, supporting the claim as “high in I”; and (ii) seniors were “high in protein” (24.8%-Soup_S and 34.0%-Balls_S of the energy was provided by proteins), “high in vitamin B12”, and had Na contents (≤0.4%) below the defined limit. All dishes reached the vitamin D target value. Sausages_C, Roulade_P, Fillet_P and Balls_S had a well-balanced protein/fat ratio. Roulade_P presented the highest n-3 PUFA/n-6 PUFA ratio (3.3), while Sausages_C the lowest SFA/UNS ratio (0.2). Dishes were considered safe based on different parameters (e.g. Hg-T, PBDEs, Escherichia coli). All represent dietary sources contributing to meet the reference intakes of target nutrients (33->100%), providing valuable options to overcome nutritional and functional imbalances of the three groups.This project has received funding from the European Union's Horizon 2020 research and innovation programme under Grant Agreement no. 773400 (SEAFOODTOMORROW). This work was also supported by the Spanish Ministry of Science and Innovation (Project CEX 2018-000794-S), the Generalitat de Catalunya (Consolidated Research Group Water and Soil Quality Unit 2017 SGR 1404) and Fundação para a Ciência e a Tecnologia (FCT)/Ministério da Ciência, Tecnologia e Ensino Superior (MCTES) through national funds (UID/QUI/50006/2019, UIDB/50006/2020, UIDP/50006/2020, UIDB/04423/2020 and UIDP/04423/2020). The authors also thank FCT and the European Union's H2020 Research and Innovation Programme for funding through the project Systemic - An integrated approach to the challenge of sustainable food systems: adaptive and mitigatory strategies to address climate change and malnutrition. Sara Cunha also acknowledges FCT for the IF/01616/2015 contract. Biotage is acknowledged for providing SPE cartridges and Bekolut for the QuEChERS kits. This output reflects the views only of the author(s), and the European Union cannot be held responsible for any use that may be made of the information contained therein

Parasitological and molecular identification of cestode Bothriocephalus scorpii sensu stricto in the new host Chelidonichthys lucerna (L. 1758) (Triglidae, Scorpaenoidei) from the North Sea

AbstractA specimen of female tub gurnard (Chelidonichthys lucerna) caught as bycatch in commercial saithe, Pollachius virens, fisheries near the Shetland Islands was identified as a new definitive host for the cestode Bothriocephalus scorpii (Bothriocephalidea) sensu stricto. It was also the first Cestoda species recorded in tub gurnard. However, as shown in our study, the identification of complex species such as Bothriocephalus scorpii based only on external characteristics had to be supplemented with molecular marker analysis to determine the taxonomic status of the specimen. Therefore, to successfully identify the parasite specimen, DNA was extracted from it, and a partial sequence of lsrDNA was amplified and the PCR product was subjected to 2 × 300 bp PE sequencing with 10,000× coverage on MiSeq. A BLAST search using the 346 bp sequence showed 100% similarity to the lsrDNA sequence of B. scorpii deposited in GenBank under accession number AF286942. Relatedness between the sequence of B. scorpii found in our study and the 20 most similar records from GenBank was identified using a phylogenetic tree constructed under maximum likelihood. Our study proves that next-generation sequencing of partial lsrDNA supplemented the morphological identification of the parasite specimen and can be applied in future parasitological studies

Impact of climate‐driven temperature increase on inland aquaculture: Application to land‐based production of common carp ( Cyprinus carpio L.)

Climate change will expose the food-producing sector to a range of challenges. Inland aquaculture farms are particularly vulnerable, due to the difficulty in changing their location, and therefore require specific tools to predict the influence of direct and indi�rect effects on production, environment and economic feasibility. The objective of our study was to apply a simple set of models to produce a set of growth, risk and suitabil�ity maps for stakeholders within the common carp sector in Poland, to assist decision�making under two different scenarios of climate change: a moderate situation (RCP 4.5) and an extreme situation (RCP 8.5). We used present (2000–2019) and future pro�jections (2080–2099) for water surface temperature based on land surface tempera�ture data from regionally downscaled climate models to draw maps to: (i) show opti�mal temperature conditions for carp growth, (ii) assess risk of disease outbreak caused by three important common carp pathogens: Cyprinid herpesvirus 3 (CyHV-3), carp oedema virus (CEV) and spring viremia of carp (SVCV) and (iii) predict potential suit�ability changes of carp farming in Poland. The study identified areas with the most and least favourable temperature conditions for carp growth, as well as those areas with the highest/lowest number of days with suitable temperatures for virus infection. These suitability maps showed the combined effect of direct and indirect effects of cli�mate change projections under RCP 8.5 and RCP 4.5 scenarios. The approach applied herein will be of use worldwide for analysing the risks of temperature increase to land�based aquaculture, and the results presented are important for carp farmers in Poland and elsewhere, industry in general, and government stakeholders, to understand the direct and indirect effects of climate change on the triple bottom line of people, planet, and profi