Cardiac hemodynamic parameters for NT and SA mice.

<p>The data is expressed as mean ± SEM (n = 6). All measurements were performed on 3 different cardiac cycles and the values averaged. BP: blood pressure; HR: heart rate; IVS: interventricular septum; LVID: left ventricle internal diameter; LVPW: left ventricle posterior wall thickness; LV Vol: left ventricle end volume; %EF: ejection fraction; %FS: <i>P</i> value (<0.05).</p>*<p>compared to NT in the same treatment and</p>#<p>compared to saline in the same genotype.</p

ACE2 over expression in the brain reduces Ang II-induced collagen deposition in aortas and coronary arteries.

<p>Mice were anesthetized and hearts were processed for Masson's Trichrome staining at the end of 2 weeks Ang II or saline infusion. The average trichrome staining densities were quantified by using Image-Pro Plus software. Brain-targeted ACE2 over expression significantly reduces collagen deposition in coronary arteries (<b>A, B</b>) and aortas (<b>C, D</b>) following 2 weeks of Ang II infusion. N = 5/group. Statistical significance: *<i>P</i><0.05 vs. NT with the same treatment; ^#<i>P</i><0.05 vs. saline with the same genotype.</p

ACE2 over expression decreases Ang II-induced left ventricle cardiac hypotrophy.

<p>2 weeks after Ang II or saline infusion, echocardiography were performed on 3 different cardiac cycles, the values averaged and used to calculate 2PW/LVD; then, mice left ventricle were collected and tibia length were measured for calculation of LV mass/Tibia ratio. ACE2 over expression significantly reduced the increase of 2PW/LVD (<b>A</b>) and LV mass/Tibia ratio (<b>B</b>) in SA compare to NT mice following Ang II infusion (P<0.05). N = 8/group. Statistical significance: *<i>P</i><0.05 vs. NT with the same treatment; ^#<i>P</i><0.05 vs. saline with the same genotype.</p

Urine NE levels.

<p>Mice were infused with Ang II or saline for 2 weeks. Urine were collected in 6 N HCl and processed for NE measurements using ELISA methods. Two weeks of Ang II infusion significantly increase urine NE levels compared to saline infusion in NT mice. However, the NE levels is significantly lower in SA compared NT at the end of 2 weeks infusion. N = 4/group. Statistical significance: *<i>P</i><0.05 vs. NT with the same treatment; ^#<i>P</i><0.05 vs. saline with the same genotype.</p