MSP-1p42 specific titers of the Ig preparations, Panel A (rabbit samples), Panel B, C (human samples) were determined by ELISA and then adjusted to the pre-purification titer

All rabbit samples were diluted 1:50 and all human samples were diluted 1:10 to achieve optimal band resolution. Lanes: 1) molecular weight marker, 2) source material (pre-purification), 3) MW marker, 4) Protein G-purified Ig, 5) Protein A/G-purified Ig, 6) CA-AS-purified Ig and 7) PEG-purified Igs. Samples in Panel A, B were tested immediately following purification. Samples in Panel C were tested for their stability after storage for 24 weeks at 4°C. Panel C, 1) MW marker, 2) Protein G-purified Ig, 3) Protein A/G-purified Ig, 4) CA-AS-purified Ig and 5) PEG-purified Igs.<p><b>Copyright information:</b></p><p>Taken from "Evaluation of immunoglobulin purification methods and their impact on quality and yield of antigen-specific antibodies"</p><p>http://www.malariajournal.com/content/7/1/129</p><p>Malaria Journal 2008;7():129-129.</p><p>Published online 14 Jul 2008</p><p>PMCID:PMC2490700.</p><p></p

Various Ig preparations from (A) rabbit sera or (B) human serum or plasma were tested for changes in the MSP-1p42 specific titer (i

E., OD = 1 at 405 nm). Data shown are the geometric mean and 95% confidence interval of three independent experiments for each purification method. Asterisks indicate statistically significant differences (p < 0.05).<p><b>Copyright information:</b></p><p>Taken from "Evaluation of immunoglobulin purification methods and their impact on quality and yield of antigen-specific antibodies"</p><p>http://www.malariajournal.com/content/7/1/129</p><p>Malaria Journal 2008;7():129-129.</p><p>Published online 14 Jul 2008</p><p>PMCID:PMC2490700.</p><p></p