Additional file 6: Figure S5. of Adhesive organ regeneration in Macrostomum lignano

RCA labelling of Macrostomum lignano. (A) Overview of a RCA stained adult animal with (A1) a confocal projection, (A2) DIC image, and (A3) overlay. Arrowheads indicate the unlabeled epidermis. (B) Detail of a tail plate with intensively labelled prostate glands and adhesive organs. (C1-3) Detail of a developing egg within the antrum. Ao adhesive organs, egg developing egg, fgo female genital opening, pg prostate glands, tp tailplate. Scale bars: (A) 100 μm, (B-C) 10 μm. (TIF 5160 kb

Additional file 3: Figure S2. of Adhesive organ regeneration in Macrostomum lignano

SBA labelling of Macrostomum lignano. (A) Overview of a SBA stained adult animal with (A1) a confocal projection, (A2) DIC image, and (A3) overlay. (B1-3) Detail of the posterior end showing the intensive labelled antrum, single stained cement glands, and the weakly stained prostate glands. (C1-3) Higher magnification of a head, revealing a dotted staining in frontal glands 1 and a ubiquitous staining in the pharyngeal gland cell bodies and frontal glands 2 and 4. An antrum, cg cement glands, fg frontal glands, pg prostate glands, ph pharyngeal glands, st stylet. Scale bars: (A) 100 μm, (B-C) 20 μm. (TIF 5721 kb

Modification of the Adhesive Properties of Silicone-Based Coatings by Block Copolymers

The improvement of the (bio)­adhesive properties of elastomeric polydimethylsiloxane (PDMS) coatings is reported. This is achieved by a surface modification consisting of the incorporation of block copolymers containing a PDMS block and a poly­(2-(dimethylamino)­ethyl methacrylate) (PDMAEMA) block in a PDMS matrix, followed by matrix cross-linking and immersion of the obtained materials in water. Contact angle measurements (CA), X-ray photoelectron spectroscopy (XPS), and atomic force microscopy (AFM) showed the presence of the PDMAEMA block at the surface, drastic morphology changes, and improved adhesion properties after immersion in water. Finally, underwater bioadhesion tests show that mussels adhere only to block copolymer-filled coatings and after immersion in water, i.e., when the PDMAEMA blocks have been brought to the coating surface. These observations highlight the significant role of hydrophilic groups in the surface modification of silicone coatings