Reference map showing localities of all barcoded material.

<p>White circle: Estuaire province (Gabon), white squares: Ogooue-Maritime province (Gabon), white triangles: Nyanga province (Gabon; LMR study site), black circles: Lekoumou department (Republic of Congo, RC), black squares: Likouala department (RC), and black triangles: Pool department (RC).</p

Maximum-likelihood phylogeny of the combined 16S and COI DNA barcode data for all specimens sequenced for this study.

<p>Family clades observed at our study site are color-coded and show a representative image. The four families shown in grey (including the caecilian) were not observed at the Loubomo-Mougagara Road study site but were included as reference material from other sites in Gabon or the Republic of Congo.</p

Loubomo-Mougagara Road (LMR) study area for amphibian biodiversity inventory in Nyanga province, Gabon.

<p>Loubomo-Mougagara Road (LMR) study area for amphibian biodiversity inventory in Nyanga province, Gabon.</p

List of amphibian species sequenced in this study.

<p>List of amphibian species sequenced in this study.</p

Number of species/morphospecies of each genus identified in the field versus identifications based on DNA barcode analyses for each sampling season and overall in the LMR study area in Gabon.

<p>Number of species/morphospecies of each genus identified in the field versus identifications based on DNA barcode analyses for each sampling season and overall in the LMR study area in Gabon.</p

Chao2 species richness estimates for each of two sampling periods at the LMR study site and the combined results using data from morphological assessments in the field (red) and from barcode analysis (blue).

<p>Error bars are 84% confidence intervals.</p

Species accumulation curves for the LMR study area in Gabon.

<p>Red lines represent species identification data from morphological assessment in the field and blue lines represent species identification data from molecular barcode operation taxonomic units for (A) the first sampling period during the dry season, (B) the second sampling period during the wet season, and (C) the combined results of both sampling periods. Dotted lines represent 84% confidence intervals.</p

bi4africa dataset - open source

The bii4africa dataset is presented in a multi-spreadsheet .ods file. The raw data spreadsheet (‘Scores_Raw’) includes 31,313 individual expert estimates of the impact of a sub-Saharan African land use on a species response group of terrestrial vertebrates or vascular plants. Estimates are reported as intactness scores - the remaining proportion of an ‘intact’ reference (pre-industrial or contemporary wilderness area) population of a species response group in a land use, on a scale from 0 (no individuals remain) through 0.5 (half the individuals remain), to 1 (same as the reference population) and, in limited cases, to 2 (two or more times the reference population). For species that thrive in human-modified landscapes, scores could be greater than 1 but not exceeding 2 to avoid extremely large scores biasing aggregation exercises. Expert comments are included alongside respective estimates

bii4africa dataset

The bii4africa dataset is presented in a multi-spreadsheet .xlsx file. The raw data spreadsheet (‘Scores_Raw’) includes 31,313 individual expert estimates of the impact of a sub-Saharan African land use on a species response group of terrestrial vertebrates or vascular plants. Estimates are reported as intactness scores - the remaining proportion of an ‘intact’ reference (pre-industrial or contemporary wilderness area) population of a species response group in a land use, on a scale from 0 (no individuals remain) through 0.5 (half the individuals remain), to 1 (same as the reference population) and, in limited cases, to 2 (two or more times the reference population). For species that thrive in human-modified landscapes, scores could be greater than 1 but not exceeding 2 to avoid extremely large scores biasing aggregation exercises. Expert comments are included alongside respective estimates