Report on a visceral and cutaneous leishmaniases focus in the town of Jequié, State of Bahia, Brazil.

Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2012-10-30T20:14:26Z No. of bitstreams: 1 A.J.Oliveira dos Santos Report on a visceral....pdf: 126541 bytes, checksum: 38044be3cdc8a50c28299f90c1dae838 (MD5)Made available in DSpace on 2012-10-30T20:14:26Z (GMT). No. of bitstreams: 1 A.J.Oliveira dos Santos Report on a visceral....pdf: 126541 bytes, checksum: 38044be3cdc8a50c28299f90c1dae838 (MD5) Previous issue date: 1993Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilSecretaria de Saúde do Estado da Bahia. Jequié, BA, BrazilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasi

American cutaneous leishmaniasis unresponsive to antimonial drugs: successful treatment using combination of N-methilglucamine antimoniate plus pentoxifylline.

Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2014-07-09T14:09:10Z No. of bitstreams: 1 Bafica A American cutaneous....pdf: 242239 bytes, checksum: 11179b434c51c965c7444dc03b6fe533 (MD5)Made available in DSpace on 2014-07-09T14:09:10Z (GMT). No. of bitstreams: 1 Bafica A American cutaneous....pdf: 242239 bytes, checksum: 11179b434c51c965c7444dc03b6fe533 (MD5) Previous issue date: 2003Universidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilCentro Referência em Doenças Endêmicas Pirajá da Silva. Salvador, BA, BrasilUniversidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilBackground American cutaneous leishmaniasis is characterized by single or multiple ulcerations. Cytokines, among other factors, have been shown to influence lesion development and tumoral necrosis factor-alpha is a major cytokine implicated in pathogenesis of ulcers. Observations We tested oral pentoxifylline, a known tumoral necrosis factor-alpha inhibitor, at doses of 400 mg (2–3x/day), associated to N-methylglucamine antimoniate (15 mg/kg/day) in two patients with American cutaneous leishmaniasis unresponsive to antimonial drugs. We observed a satisfactory response with quick cure of skin lesions of these patients. Conclusions Our results suggest that oral pentoxifylline in association to N-methylglucamine antimoniate should be consider in refractory cutaneous leishmaniasis patient

Assessment of an optimized dog-culling program in the dynamics of canine Leishmania transmission.

Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2011-10-26T20:50:17Z No. of bitstreams: 1 Moreira ED Jr Assessment of an optimized....pdf: 67414 bytes, checksum: c7ddfccb579637915478d942ed439784 (MD5)Made available in DSpace on 2011-10-26T20:50:17Z (GMT). No. of bitstreams: 1 Moreira ED Jr Assessment of an optimized....pdf: 67414 bytes, checksum: c7ddfccb579637915478d942ed439784 (MD5) Previous issue date: 2002Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversity of California at Berkeley. School of Public Health. Berkeley, USASecretaria da Saúde do Estado da Bahia. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilIn Brazil, zoonotic visceral leishmaniasis (ZVL) control programs based on the mass elimination of seropositive dogs have failed to reduce the number of leishmaniasis cases. However, these programs have been done under sub-optimal conditions. We studied a cohort of dogs in an urban area in Brazil to determine, whether a dog-culling program optimized with: (i) replacement of a relatively low-sensitivity indirect immune-fluorescent test on blood eluate by a more sensitive enzyme-linked immunosorbent assay on serum blood samples; (ii) shortening of the time interval from serodiagnosis to removal of dogs; (iii) screening a high proportion of the dog population could reduce the incidence of canine Leishmania infection (CLI). The study ran from December 1997 to July 2000, with four follow-up assessments performed at approximately 8-month intervals. All dogs seropositive for anti-Leishmania antibodies were promptly eliminated. A large number of new dogs immigrated to the study area throughout the study period. They comprised 43.8–49.8% of the cohort at each follow-up assessment, and upto 15% of them already had Leishmania infection. Overall, 42 news cases of CLI were identified, for a crude incidence rate of 11.8 cases per 100 dog-years (95% CI 8.6–15.6). In the first, second, third and fourth follow-up assessments the incidence rates were 8.2 (95% CI 3.0–17.9), 12.2 (95% CI 6.3–21.2), 16.4 (95% CI 8.5–28.6) and 13.

Skin reactions to thimerosal and Leishmania in dogs from a leishmaniasis endemic area: it is better to keep them apart

Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2012-09-19T18:02:35Z No. of bitstreams: 1 Paranhos-Silva M Skin reactions to thimerosal ....pdf: 39559 bytes, checksum: b4a024360585e65fe8c15bb1836af99d (MD5)Made available in DSpace on 2012-09-19T18:02:35Z (GMT). No. of bitstreams: 1 Paranhos-Silva M Skin reactions to thimerosal ....pdf: 39559 bytes, checksum: b4a024360585e65fe8c15bb1836af99d (MD5) Previous issue date: 2001Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilCentro de Referência em Doenças Endêmicas Pirajá da Silva. Jequié, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilPositive Montenegro's skin test is a delayed type hypersensitivity reaction widely used as indicative of previous infection with Leishmania in both humans and dogs. Montenegro's antigen consists of a crude Leishmania antigen solution, usually containing thimerosal as preserving agent. In this work it is shown that a large proportion of dogs (11 out of 56) examined in an endemic area of leishmaniasis presented induration at the site of injection of a diluent containing thimerosal alone. This clearly demonstrates that thimerosal leads to a high number of false positive skin reactions in dogs and that its use in Montenegro's skin test antigenic preparations should be avoide

Anti-beta2 glycoprotein I and anticardiolipin antibodies in leptospirosis, syphilis and Kala-azar.

Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2015-10-14T17:09:26Z No. of bitstreams: 1 Santiago, M Anti-beta 2 glycoprotein I....pdf: 356814 bytes, checksum: 95b6dbbab2ab8b46eceb37019dcbe270 (MD5)Approved for entry into archive by Ana Maria Fiscina Sampaio ([email protected]) on 2015-10-14T17:34:49Z (GMT) No. of bitstreams: 1 Santiago, M Anti-beta 2 glycoprotein I....pdf: 356814 bytes, checksum: 95b6dbbab2ab8b46eceb37019dcbe270 (MD5)Made available in DSpace on 2015-10-14T17:34:49Z (GMT). No. of bitstreams: 1 Santiago, M Anti-beta 2 glycoprotein I....pdf: 356814 bytes, checksum: 95b6dbbab2ab8b46eceb37019dcbe270 (MD5) Previous issue date: 2001Fundação Baiana para o Desenvolvimento da Ciência. Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil / Hospital Santa Izabel. Salvador, BA, BrasilUniversidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Cornell University. Weill Medical College. New York, NYFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilCOAS. DST. SESAB. Salvador, Bahia, BrasilPIEJ. SESAB. Salvador, Bahia, BrasilMorehouse School of Medicine. Atlanta, Georgia, USAMorehouse School of Medicine. Atlanta, Georgia, USAMorehouse School of Medicine. Atlanta, Georgia, USAReports have shown that anticardiolipin (aCL) antibodies present in patients with autoimmune diseases are dependent on the cofactor,beta2 glycoprotein I (beta2 GPI), as opposed to aCL antibodies seen in infectious diseases such as syphilis, HIV hepatitis C, etc. The assay for anti-beta2GPI antibodies has been reported to be more specific for antiphospholipid syndrome (APS). However, the prevalence of these antibodies in diseases such as leishmaniasis and leptospirosis remains unknown. The aim of the present study was determine the prevalence of antibodies to cardiolipin and to beta2GPI in patients with different infectious diseases, including leptospirosis, syphilis and leishmaniasis. METHODS: Samples from patients with Kala-azar (visceral leishmaniasis), syphilis or leptospirosis were tested for IgG and IgM anticardiolipin and IgG anti-beta2GPI antibodies by ELISA. RESULTS: In patients with Kala-azar the prevalence of IgG aCL, IgM aCL and anti-beta2GPI was 6% (2/30), 3% (1/30) and 53% (16/30), respectively. In syphilis the prevalence was 18% (14/74), 13% (10/74) and 10% (8/70), respectively. In leptospirosis the frequency of these antibodies was 23% (9/39), 10% (4/39) and 17% (6/34), respectively. There was no statistical correlation between aCL and anti-beta2GPI antibodies in these diseases. DISCUSSION: This study clearly shows a significant prevalence of anti-beta2GPI antibodies in leptospirosis and leishmaniasis and syphilis. This indicates that the assay for antibeta2GPI antibodies should be thoroughly validated before it is introduced as a definitive tool for the diagnosis of APS, testing a larger number of sera from patients with a wider range of clinical conditions

Montenegro’s skin reactions and antibodies against different Leishmania species in dogs from a visceral leishmaniosis endemic area

Agradecimentos ao Dr.Edson Duarte Moreira pela sua revisão cuidadosa da estatística e análise dos dados aqui apresentados.Submitted by Éder Freyre ([email protected]) on 2011-08-16T14:39:46Z No. of bitstreams: 1 Baleeiro_ParanhosSilva_Santos_etal.pdf: 107901 bytes, checksum: 23553d8d338511ff9f359734ef194bc2 (MD5)Made available in DSpace on 2011-08-16T14:39:46Z (GMT). No. of bitstreams: 1 Baleeiro_ParanhosSilva_Santos_etal.pdf: 107901 bytes, checksum: 23553d8d338511ff9f359734ef194bc2 (MD5) Previous issue date: 2006Este estudo foi financiado pelo CNPq e pela FAPESB.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilLaboratório Central do Estado da Bahia (LACEN). Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilCentro de Referência em Endemias Pirajá da Silva (PIEJ), Jequie, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, BrasilIn this study, humoral (circulating anti-Leishmania antibodies) and cellular (Montenegro's skin test) immune responses of dogs from an endemic area of visceral leishmaniosis were tested using Leishmania chagasi, Leishmania amazonensis and Leishmania braziliensis antigens. The antibody response was tested in three animal groups, selected according to their anti-L. chagasi antibody activity, as measured by ELISA in the serum: 19 negative (O.D. below 0.30), seven with undefined (O.D. between 0.40 and 0.70) and 12 positive (O.D. above 1.0) ELISA result. In the group of animals with positive ELISA, the antibody activity against L. chagasi antigens (mean O.D.=1.31) was significantly higher (ANOVA, P<0.01) than against L. amazonensis (mean O.D.=0.88) or L. braziliensis (mean O.D.=0.87) antigens. The Montenegro's skin test results obtained with L. chagasi and L. braziliensis antigens showed a fair agreement (kappa=0.309). The same was observed when antigens from L. braziliensis and L. amazonensis were compared (kappa=0.374), whereas a moderate agreement between the results from tests performed with L. chagasi and L. amazonensis antigens was observed (kappa=0.530). The induration areas obtained with L. braziliensis antigen were smaller than those obtained with the other antigens. The data presented herein indicate that the use of antigens from different Leishmania species may interfere with the results of the immunological tests performed in dogs in an endemic area of visceral leishmaniosis

Cohort study on canine emigration and Leishmania infection in an endemic area for American visceral leishmaniasis. Implications for the disease control.

Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2014-05-28T18:23:22Z No. of bitstreams: 1 Paranhos-SILVA M Cohort study on canine....pdf: 283577 bytes, checksum: 8eb65fd7af1df62137a923390c4b7ceb (MD5)Made available in DSpace on 2014-05-28T18:23:22Z (GMT). No. of bitstreams: 1 Paranhos-SILVA M Cohort study on canine....pdf: 283577 bytes, checksum: 8eb65fd7af1df62137a923390c4b7ceb (MD5) Previous issue date: 1998Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, BrasilSecretaria de Saúde do Estado da Bahia. 13a DIRES. Jequié, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilAmerican visceral leishmaniasis is a main public health matter in Brazil. Since dogs have been incriminated as the main urban reservoir of AVL agent Leishmania chagasi, a cohort study aimed at understanding the dynamics of the canine infection was carried out in Jequie´—an endemic community in the Northeast of Brazil. The inhabited urban and periurban areas of Jequie´ were divided into 140 clusters of 0.25 km2. All 1681 dogs domiciled in 34 randomly selected clusters were screened for Leishmania antibodies in an enzyme-linked immunosorbent assay. After the seropositive dogs were painlessly eliminated, a cohort of 1286 seronegative dogs was followed up for 18 months, yielding a total of 1739.7 dog-years. The overall incidence of Leishmania infection, as assessed by the detection of Leishmania antibodies in blood samples collected every six months, was 6.55 cases:100 dog-years (95% confidence interval; CI 6.04–7.26). Two subsets of clusters, with 0.70 and 1.35 relative risks of infection, were identified. The annual emigration rate was 2.26 cases:100 dog-years (95% CI 1.86–2.66). The implications of these findings for the control of American visceral leishmaniasis are discussed

Clinical utility of polymerase chain reaction-based detection of Leishmania in the diagnosis of American cutaneous leishmaniasis.

Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2014-07-09T13:53:09Z No. of bitstreams: 1 Oliveira CI Clinical utility....pdf: 108313 bytes, checksum: a0806eadf613e91fe4ca5607f9f9f6c3 (MD5)Made available in DSpace on 2014-07-09T13:53:09Z (GMT). No. of bitstreams: 1 Oliveira CI Clinical utility....pdf: 108313 bytes, checksum: a0806eadf613e91fe4ca5607f9f9f6c3 (MD5) Previous issue date: 2003Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, BrasilCentro de Referência para Doenças Endêmicas Pirajá da Silva. Jequié, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, BrasilWe evaluated the use of polymerase chain reaction (PCR) for diagnosis of American cutaneous leishmaniasis (ACL) in an area in Bahia, Brazil, where Leishmania braziliensis is endemic. Leishmania DNA was detected in 50 cases, yielding a positivity rate of 100%, which was higher than the rates for all of the other diagnostic methods studied--namely, the Montenegro skin test, anti-Leishmania serological testing, and microscopic examination of lesion biopsy specimens. These findings have led us to propose guidelines for the diagnosis of ACL that use PCR as the principal means of parasitological confirmation of case