Electrophoretic profiles and antibody labelling of PrP^res in BSE-derived prions.

<p>PrP^res was detected by western blot using the mAbs Sha31 (A) and 12B2 (B) in the different BSE-derived prions propagated either in natural hosts or in transgenic mouse models over-expressing their corresponding PrP^C sequence: cattle-BSE (lanes 1 and 11), BoTg-BSE (lanes 2 and 12), sheep-BSE (lane 3), OvTg-BSE (lane 4), pig-BSE (lane 5), PoTg-BSE (lane 6), mouse-BSE (lane 7), MoTga20-BSE (lane 8), human-vCJD (lane 13), HuTg-BSE (lane 14). Sheep-scrapie (lane 9), mouse-RML (lane 10), human-sCJD (lane 15) and atypical cattle-BSE H (lane 16) were included as control non-BSE related prions. Panels A and B were loaded with the same quantities of PrP^res extracted from each sample. MW, molecular weight in kilodaltons.</p

PrP^res in BoPrP-Tg110 mice.

<p>Electrophoretic profiles and antibody labelling of PrP^res as detected by mAbs Sha31 (A) and 12B2 (B) in brain extracts from BoPrP-Tg110 mice inoculated with the different BSE-derived prions: cattle-BSE (lane 1), sheep-BSE (lane 2), OvTg-BSE (lane 3), pig-BSE (lane 4), PoTg-BSE (lane 5), mouse-BSE (lane 6), MoTga20-BSE (lane 7), human-vCJD (lane 8), HuTg-BSE (lane 9). Brain extracts from BoPrP-Tg110 mice inoculated with atypical cattle-BSE H (lane 10), sheep-scrapie (lane 11) and mouse-RML (lane 12) were included as a control non-BSE related prion propagated in the same mouse model. Panels A and B were loaded with the same quantities of PrP^res extracted from each sample. MW, molecular weight in kilodaltons.</p

Glycoform ratios of PrP^res detected by Western blotting.

<p>PrP^res was detected with the Sha31 monoclonal antibody in the different BSE-derived prions propagated either in the natural hosts (A) or in transgenic mouse models over-expressing their corresponding PrP^C sequence (B). Atypical cattle-BSE H, sheep-scrapie, mouse-RML and human-sCJD isolates (C) are included for comparison purposes. Values are the normalized means from at least six repeated runs. Arrows indicate the reading direction of the axis.</p

PrP^res in HuPrP-Tg340 mice.

<p>Electrophoretic profiles and antibody labelling of PrP^res as detected by mAbs Sha31 (A) and 12B2 (B) in brain extracts from HuPrP-Tg340 mice inoculated with the different BSE-derived prions: cattle-BSE (lane 1), BoTg-BSE (lane2), sheep-BSE (lane 3), OvTg-BSE (lane 4), pig-BSE (lane 5), PoTg-BSE (lane 6), mouse-BSE (lane 7), MoTga20-BSE (lane 8), human-vCJD (lane 9), HuTg-BSE (lane 10). Brain extract from HuPrP-Tg340 mice inoculated with human-sCJD isolate (lane 11) was included as a control non-BSE related prion propagated in the same mouse model. Panels A and B were loaded with the same quantities of PrP^res extracted from each samples. MW, molecular weight in kilodaltons.</p

PrP^Sc proteinase K resistance ELISA test in BSE-derived prions.

<p>A) BSE-derived prions propagated in several host species (cattle-BSE, sheep-BSE, pig-BSE, mouse-BSE and human-vCJD); mouse-RML, sheep-scrapie and human-sCJD isolates are included for comparison purposes. B) BSE-derived prions propagated in transgenic mice expressing PrP^C of these species (BoTg-BSE, OvTg-BSE, PoTg-BSE, MoTga20-BSE and HuTg-BSE).</p

Transmission features of BSE-derived prions in comparison with non-BSE related prions in different mouse models (BoPrP-Tg110, PoPrP-Tg001 and HuPrP-Tg340).

a<p>Intracerebral inoculation with 2 mg brain tissue equivalent; n/n₀: diseased, PrP^res positive/inoculated animals; SEM: standard error of the mean.</p><p>ND: not determined.</p><p>Results from homologous transmissions (host and donor share the same species-PrP sequence) are marked in bold.</p