Oleiculture in progress

The present work evaluates the limits and possibilities of development with regard to the most recent olive-growing techniques in light of up-to-date knowledge of species characteristics. After a brief introduction regarding the productive capacity of olive, the new taxonomic position of the cultivated species and a reorganization of the genus Olea is presented in the first part of the work. Examination follows of the assumed stages of domestication, spread (from the Bronze Age until decline in the 6-10th centuries A.D.) and then globalization of the species from the 19th century until the present. The second part addresses the spread of olive to the different continents, environmental limitations to its cultivation and the growth model that distinguishes it from most of the other cultivated woody species. The various problems that arise when olive is cultivated outside its areal of origin are considered, from induction processes to effective chilling requirements, as well as the effects of climatic environment on plant growth and product quality following shifts in areal. The paper concludes with a brief analysis of open questions relative to new models of cultivation

Il fiore e la biologia fiorale

Floral biology includes the series of processes which leads buds to evolve into flowers and fruits. Improving the knowledge on floral biology in olive is important both for scientific understanding and for practical reasons, since optimizing these applications results in better fruit yield. These processes are: induction (the conversion of vegetative to inflorescence buds), differentiations (the actual formation of flower parts in the bud), floral formation (the formation and evolution of the inflorescence and the flowers after bud brake), flowering (timing, flower types, andromonoecy), pollination, fertilization and compatibility, ovary abortion, fruit set. This papers describes all these steps and discusses the most recent knowledge on olive floral biology, including some recent theories like the “aging” theory, for which a minimum aging of the meristematic apex is necessary to allow floral induction and differentiation, and the “competition” theory, for which the greater competition for resources among flowers in cultivars with larger flower and fruit sizes, explains the greater ovary abortion and reduced fruit set in such cultivars. Finally, the very low fruit set and the apparently redundant flowering in olive is explained in evolutionary terms (mail fitness). The paper ends with concluding remark and practical suggestions for improving fruit yield, and provides answers to frequently asked questions.Realizzato nell'ambito del progetto "Ricerca ed Innovazione per l'Olivicoltura Meridionale", finanziato dal MiPAA

A new device to improve the mechanical winter pruning in olive trees hedgerows

The economic success of superintensive olive plantations is mainly due to the full mechanization of the harvesting and pruning. While the advantage of straddling machines is undoubted, winter mechanical pruning determines falls in productions. This is due to the indiscriminate suppression of both fertile leafy shoots destined to fruiting and growth, and exhausted parts of the plant. To reduce this damage, an innovative device has been developed, applied to a pruning machine, able to selectively cut the “aged” parts of vegetation. The selection is achieved by an air’s fluid dynamic action obtained throughout defined and directional air jets able to push the young and flexible shoots upwards and downwards; in this way they are saved by the cut, regenerating at least one year in advance the new fruiting hedge. Tests were carried out on the cultivar Arbequina, Tosca and Sikitita, in three superintensive olive groves located in the province of Grosseto, Rome and Latina, assessing the amount of leaves, shoots and branches, as well as fruits present at harvest, preserved from the pruning thanks to the action of the air flow, respectively for the East and West side of the rows. To get a profile of the biomass distribution along the cross section of the tree canopy, in the Grosseto farm a trial was also carried out to better assess leaves, shoots and branches distribution in the canopy. The statistical data analyses immediately evidenced two different populations due to the selective pruning. The work highlighted the remarkable effectiveness of the air jet in safeguarding the flexible and leafy vegetation and allowed to quadruple leaf surface and production

Kinetics of volatile organic compounds (VOCs) released by roasted Coffee during the first ten days after processing

The quality of coffee is linked to the aroma created by the chemical reactions that occur during the roasting process. While it is generally thought that roasted coffee is a stable product with a relatively long shelf-life, little information is available on the evolution (kinetic) of the volatile organic compounds (VOCs) in the days immediately following the process. The aim of this study is to determine the evolution of VOCs released by coffee beans, on samples of Coffea arabica (three different origins) and Coffea canephora (1 single origin), by using a Proton Transfer Reaction Time-of-Flight Mass Spectrometer (PTR-ToF-MS) 24 hours after roasting, and for the next 9 days. Results confirmed the differences already highlighted in previous studies between the VOCs spectra of the two species. There were also significant differences in the intensity of emissions for the different origins of Coffea arabica, with the highest VOCs amount over time always detected in the Honduras Arabica samples. The involved detected protonated ions were grouped into three classes: compounds (ppbv) present with decreasing quantity ; weakly increasing; almost constant trend; or always increasing. A complex dynamic emerged for the different protonated ions over time, which not only affects the mass spectra of the different species but also influences the configuration of the mass spectra of the different geographical zones of production

Monitoring in real time the changes in VOCS emission in sunflower and extra virgin olive oil upon heating by PTR-TOF-MS

In this work the emission of volatile organic compounds (VOCs) upon the heating process of an extra virgin olive oil (EVOO) and a high oleic sunflower oil (SFO) was evaluated in real time by spectrometry. Two tests were carried out, in the first VOCs emitted from both kinds of oil were measured at room temperatures (not heated, NH) and at 180°C; in the second test, VOCs emission for selected masses were monitored under increasing temperatures over time: at room temperature not heated oils (NH), 60, 90, 120, 150, and 180°C, respectively. The spectra were acquired using a Proton Transfer Reaction Time of Flight Mass Spectrometer (PTR-ToF-MS). The total VOCs emission increased at 180°C, determined both by the rise of the amount of compounds present in the NH samples and by the formation of new masses generated by oxidative chemical reaction from triglycerides and fatty acids. From the set of results it is evident that a good control of the temperatures can be useful in reducing the quantities of masses potentially harmful to health in human food

Comparative analysis of Volatile Compounds (potential aromatic ability) in the fruit of 15 olive Italian cultivars

Virgin olive oils (VOOs) are characterized by peculiar flavors appreciated by the consumers all over the world. Their organoleptic characteristics depend on the aromatic properties of the fruits of the different cultivars, which will originate the final products. VOCs spectra of fifteen certified Italian olive cultivars of the University of Florence Germplasm collection, chosen as their different geographical origin, diffusion, and product purpose, were acquired using a Proton Transfer Reaction Time-of-Flight Mass Spectrometer (PTR-ToF-MS). The VOCs analyses highlighted a great variability among the fifteen cultivars, mostly due to compounds (C6 and C5) deriving from polyunsaturated fatty acids through the LOX pathway. The early identification in the olive fruit of these compounds which are considered among the major contributors to the positive VOOs attributes, would be useful to produce high quality olive oils, and get useful information to individuate the best parents for the genetic improvement.

Volatile compounds from different fruit parts of two cultivars of Cydonia oblonga

Quince is characterized as a fragrant fruit which, unlike other pomes (apple, pear), is not used for fresh consumption due to its astringency and compactness, but only in its processed form (jams, jelly, distillery products, and nutraceutical compounds). As a consequence, there is little knowledge currently available concerning the characteristics of the fruit, and in particular its aromatic and chemotaxonomic patterns. In this work, carpometric, chemometric and spectrophotometric measurements were performed on quince fruits. VOCs emitted by different tissues or parts of the fruit were studied to describe its aromatic profile. The study was carried out on the fruits of an old, well-known cultivar (‘Gigante di Wranja’, commonly called ‘Wranja’) and a new Tuscan accession. Intact, halved and solely pulp (cubed) samples were evaluated for each individual fruit. Data obtained from VOC analysis through Proton Transfer Reaction Time-of-Flight Mass Spectrometer (PTR-ToF-MS) were evaluated by multivariate statistical analysis. The spectra obtained from the intact fruit samples showed a higher amount of masses corresponding to terpenes or terpenoid compounds, which fundamentally characterize the aroma of this type of fruit; these substances were found to be much less present in the VOCs emitted by the pulp, where high values of masses linked to the maturation processes were instead found

Tuberomics: a molecular profiling for the adaption of edible fungi (Tuber magnatum Pico) to different natural environments

Background: Truffles are symbiotic fungi that develop underground in association with plant roots, forming ectomycorrhizae. They are primarily known for the organoleptic qualities of their hypogeous fruiting bodies. Primarily, Tuber magnatum Pico is a greatly appreciated truffle species mainly distributed in Italy and Balkans. Its price and features are mostly depending on its geographical origin. However, the genetic variation within T. magnatum has been only partially investigated as well as its adaptation to several environments. Results: Here, we applied an integrated omic strategy to T. magnatum fruiting bodies collected during several seasons from three different areas located in the North, Center and South of Italy, with the aim to distinguish them according to molecular and biochemical traits and to verify the impact of several environments on these properties. With the proteomic approach based on two-dimensional electrophoresis (2-DE) followed by mass spectrometry, we were able to identify proteins specifically linked to the sample origin. We further associated the proteomic results to an RNA-seq profiling, which confirmed the possibility to differentiate samples according to their source and provided a basis for the detailed analysis of genes involved in sulfur metabolism. Finally, geographical specificities were associated with the set of volatile compounds produced by the fruiting bodies, as quantitatively and qualitatively determined through proton transfer reaction-mass spectrometry (PTR-MS) and gas-chromatography mass spectrometry (GC-MS). In particular, a partial least squares-discriminant analysis (PLS-DA) model built from the latter data was able to return high confidence predictions of sample source. Conclusions: Results provide a characterization of white fruiting bodies by a wide range of different molecules, suggesting the role for specific compounds in the responses and adaptation to distinct environments

Quadro 8.1. Sintomatologia della disaffinità di innesto

Il Quadro si riferisce ad alcuni aspetti particolari della affinità/disaffinità di innesto, con specifico riferimento al genere "Vitis". Questo trattato di Arboricoltura generale, che rappresenta lo sforzo collegiale di 44 Autori, è rivolto prevalentemente agli studenti che seguono i corsi di laurea universitari nel settore dell'arboricoltura da frutto (inclusi vite, agrumi ed olivo) e contiene numerosi riferimenti ed esempi riguardanti gli alberi ornamentali, forestali e di ambiente urbano. L'opera è diretta principalmente alle lauree triennali di 1° livello, ma il contenuto programmatico è arricchito di specifici approfondimenti sia nel testo sia sotto forma di "quadri", che sono di fatto delle anticipazioni utilizzabili anche per le successive lauree magistrali di 2° livello o per i corsi di Master