Sequence alignment between dog, cat, human, mouse and rat specific glutamine-rich regions located in exon 3 and 11 of TET2 gene.

<p>The image was obtained using the tool ClustalW2 (<a href="http://www.ebi.ac.uk/Tools/msa/clustalw2/" target="_blank">http://www.ebi.ac.uk/Tools/msa/clustalw2/</a>).</p

List of genetic variations grouped for gene, relative population frequency and allelic frequencies in the MCT cohort of samples.

<p>List of genetic variations grouped for gene, relative population frequency and allelic frequencies in the MCT cohort of samples.</p

Association between the number of glutamine (Gln) repetitions and healthy/pathologic state in 114 canine blood and MCT samples.

<p>Pearson χ² test (p = 0.3454; not significant).</p

List of genetic variations detected in the glutamine rich region of TET2 exon 11 with relative population frequency and total glutamine residues number in the 75 MCT samples.

<p>List of genetic variations detected in the glutamine rich region of TET2 exon 11 with relative population frequency and total glutamine residues number in the 75 MCT samples.</p

Forward (F) and reverse (R) primer sequences of canine genes included in the present study and used for polymerase chain reaction with the corresponding annealing temperature and product length.

<p>Forward (F) and reverse (R) primer sequences of canine genes included in the present study and used for polymerase chain reaction with the corresponding annealing temperature and product length.</p

List of target genes and percentage of protein sequence identity between dog and other reference species (<i>Homo sapiens</i>, <i>Felis catus</i>, <i>Mus musculus</i>, <i>Rattus norvegicus</i>).

<p>NA: sequence not available in the databases.</p><p>List of target genes and percentage of protein sequence identity between dog and other reference species (<i>Homo sapiens</i>, <i>Felis catus</i>, <i>Mus musculus</i>, <i>Rattus norvegicus</i>).</p

The human and canine promoter sequences show species-differences.

<p>Alignment of human G-quadruplex sequences and putative canine sequences identified by cloning and sequencing. A) d_kit1; B) d_kit2; C) d_kit2_A16.</p

Guanine mutations within kit2 sequences alter electrophoretic mobility of folded forms.

<p>Electrophoretic mobility of human and canine kit2 sequences previously annealed at 4 or 40 µM strand concentration in 10 mM Tris, 50 mM KCl, pH 7.5.</p

Melting temperatures of the tested sequences determined from the analysis of the variation of the CD signal recorded at 260°C/min was applied.

<p>*values determined at 295 nm.</p

The identified (SNP) in canine kit2 is not associated to the healthy/MCT state.

<p>Association between the presence of d_kit2 or d_kit2_A16 and healthy/pathologic state in 51 canine blood and tumor samples. Fisher exact test (p = 0.1577).</p