Gender and Regional Differences in Body Image Dissatisfaction in Modern University Students: A Pilot Study in Two Cities of Eastern Europe

Complex anthropological and psychological study of the university students was carried out in two regions (the cities of Moscow and Tiraspol) with the aim to reveal gender and regional differences of body image dissatisfaction and their connection with the body build. 502 individuals (187 males and 315 females) aged from 17 to 25 years were investigated. The program included anthropometric measurements (height and weight, with further calculation of Body Mass Index – BMI), evaluation of body mass components, as well as psychological testing with Stunkard’s silhouette scale and the Situational Inventory of Body-Image Dysphoria (SIBID). It was found that among the representatives of both sexes the level of dissatisfaction with their own body is relatively similar (69% of males and 67% of females). However the girls were mostly dissatisfied with their excessive, as they perceived, body mass (83% of the total number of dissatisfied individuals) while the boys were dissatisfied mostly because of their underweight (60% of the total number of dissatisfied individuals). Besides, the girls were more likely to exaggerate their weight while the boys perceived it as smaller than it really was. In girls certain social influences had more impact on body image dissatisfaction than in boys. Among the girls studied, the Muscovites were more critical to their own physical appearance, which resulted in lower self-assessment of their body image and, consequently, in less positive influence of this assessment on the quality of life compared to the girls from Tiraspol

Model-Based Analysis of Changes in the Morphological Characteristics of Moscow Students for the Last Two Decades

The aim of the paper is to develop a model of changes in morphological characteristics of young males and females body structure at the beginning of the 21st century. For this purpose the results of Moscow students’ annual screenings from 2000 to 2018 were analyzed (total number of 17–18-year-olds − 6,433 individuals). As a result, five factors describing the trends of changes were revealed and further analysis demonstrated variants of their values over time. An original model was developed, which reflected real morphological transformations in the body parameters of the young generation for the last 20 years. For both sex groups a trend towards macrosomia (gr. macros − big, soma- body) was revealed. For the boys some increase in linear traits was found with a decrease in muscularity. In girls, parabolic association between athletic characteristics and the year of investigation was found: the decrease in muscularity for the first decade, and its increase for the second one

The Role of Cyclomodulins and Some Microbial Metabolites in Bacterial Microecology and Macroorganism Carcinogenesis

A number of bacteria that colonize the human body produce toxins and effectors that cause changes in the eukaryotic cell cycle—cyclomodulins and low-molecular-weight compounds such as butyrate, lactic acid, and secondary bile acids. Cyclomodulins and metabolites are necessary for bacteria as adaptation factors—which are influenced by direct selection—to the ecological niches of the host. In the process of establishing two-way communication with the macroorganism, these compounds cause limited damage to the host, despite their ability to disrupt key processes in eukaryotic cells, which can lead to pathological changes. Possible negative consequences of cyclomodulin and metabolite actions include their potential role in carcinogenesis, in particular, with the ability to cause DNA damage, increase genome instability, and interfere with cancer-associated regulatory pathways. In this review, we aim to examine cyclomodulins and bacterial metabolites as important factors in bacterial survival and interaction with the host organism to show their heterogeneous effect on oncogenesis depending on the surrounding microenvironment, pathological conditions, and host genetic background

A Large-Scale Allosteric Transition in Cytochrome P450 3A4 Revealed by Luminescence Resonance Energy Transfer (LRET)

<div><p>Effector-induced allosteric transitions in cytochrome P450 3A4 (CYP3A4) were investigated by luminescence resonance energy transfer (LRET) between two SH-reactive probes attached to various pairs of distantly located cysteine residues, namely the double-cysteine mutants CYP3A4(C64/C468), CYP3A4(C377/C468) and CYP3A4(C64/C121). Successive equimolar labeling of these proteins with the phosphorescent probe erythrosine iodoacetamide (donor) and the near-infrared fluorophore DY-731 maleimide (acceptor) allowed us to establish donor/acceptor pairs sensitive to conformational motions. The interactions of all three double-labeled mutants with the allosteric activators α-naphthoflavone and testosterone resulted in an increase in the distance between the probes. A similar effect was elicited by cholesterol. These changes in distance vary from 1.3 to 8.5 Å, depending on the position of the donor/acceptor pair and the nature of the effector. In contrast, the changes in the interprobe distance caused by such substrates as bromocriptine or 1-pyrenebutanol were only marginal. Our results provide a decisive support to the paradigm of allosteric modulation of CYP3A4 and indicate that the conformational transition caused by allosteric effectors increases the spatial separation between the beta-domain of the enzyme (bearing residues Cys₆₄ and Cys₃₇₇) and the alpha-domain, where Cys₁₂₁ and Cys₄₆₈ are located.</p></div

Interactions of double-labeled CYP3A4 proteins with testosterone monitored by LRET.

<p>Interactions of C377/C468-DY/ER (circles) and C58/C64/C121-ER/DY (triangles) with testosterone monitored by LRET in steady-state (<i>a</i>) and lifetime (<i>b</i>) setup. Panel <i>a</i> shows the changes in the intensity of donor fluorescence observed in titrations of the double-labeled proteins with testosterone. The lines show the results of fitting of the data sets to <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0083898#pone.0083898.e004" target="_blank">Eq. 4</a>. Panel <i>b</i> shows the phosphorescence decay traces recorded in the absence of added ligand (open symbols) and in the presence of 100 µM testosterone (closed symbols)</p

Parameters of ligand-induced spin shift in CYP3A4 and its unlabeled and double-labeled mutants^*.

<p>*The values given in the table represent the averages of 2–7 individual measurements, and the ± values show the confidence interval calculated for <i>p</i> = 0.05.</p