Bacterial contamination of white coats and hands of healthcare workers at mansoura university children’s hospital, Mansoura-Egypt

Background: Transmission of hospital acquired infections (HAIs) may be associated with contamination of healthcare workers’ (HCWs) hands and white coats.Objective: The purpose of this study was to clarify the role of HCWs’ white coats in transmitting HAIs and to determine the association between bacterial contamination of HCWs’ hands and white coats.Methods: A total of 154 HCWs were enrolled in the study; different samples were taken from their hands and white coats. Samples were processed and both microbiological and biochemical characterization of the isolates were done using standard microbiological protocols.Results: Up to 65.6% of hands and 61% of coats of HCWs were contaminated by microorganisms. Staphylococcus aureus was the most commonly isolated organisms from both hands and coats of HCWs (29.2%, 27.3% respectively) followed by MRSA (22.1%, 24.7% respectively).Conclusions: The risk for contamination of hands and coats of HCWs is high in different clinical settings. In order to reduce the rate of HAIs, a strict dress protocol should be set into play to prevent cross contamination between HCWs and patients.Keywords: contamination, HCWs, coat, hand, Staphylococcus aureus, MRSA. Contamination bacterienne de manteaux blancs et de mains de soins de sante a l'hopital pour enfants de l'universite mansoura, Mansoura-EgypteContexte: La transmission des infectionsObjectif: Le but de cette étude était de déterminer l'étendue, le type et l'association entre la contamination bactérienne des mains des travailleurs de la santé et les blouses blanches.Méthodes: Au total, 154 travailleurs de la santé ont été inclus dans l'étude; différents échantillons ont été pris de leurs mains et des manteaux blancs. Les échantillons ont été traités et la caractérisation microbiologique et biochimique des isolats a été effectuée en utilisant des protocoles microbiologiques standard.Résultats: Jusqu'à 65,6% des mains et 61% des couches de TS ont été contaminées par des micro-organismes. Le Staphylococcus aureus était le plus souvent isolé des deux mains et des deux sexes (29,2%, 27,3%), suivi par le SARM (22,1%, 24,7% respectivement).Conclusions: Le risque de contamination des mains et des couches de TS est élevé dans différents contextes cliniques. Afin de réduire le taux d'IASS, un protocole vestimentaire strict devrait être mis en place pour prévenir la contamination croisée entre les travailleurs de la santé et les patients.Mots clés: contamination, agents de santé, manteau, main, Staphylococcus aureus, SAR

Clinical and microbiological characteristics of healthcare-associated infections in a tertiary care pediatric hospital

Background and aim: Healthcare-associated infections (HAIs) are an important cause of prolonged hospital stay. This study was conducted to assess the incidence, causative organisms and risk factors of HAIs in pediatric patients. Subjects and methods: Our study was a prospective evaluation of Healthcare-associated infections (HAI) in patients admitted to Mansoura University Children’s Hospital, Mansoura, Egypt over a period of one year. Results: Five-hundred twenty-nine patients were included in the study. The overall rate of HAI was 5.2/100 admissions. Infection rates were highest in Pediatric ICU (25.9/100 admissions). The most frequent sites of HAI in our study were BSI (154 cultures; 33.3%), UTI (152 cultures; 32.9%), and VAP (90 cultures;19.5%). Gram negative bacteria were the most common bacteria isolated from positive cultures (47.6% of total 462 positive cultures) and were found to be multidrug resistant bacteria. A high prevalence of Methicillin-resistant Staphylococcus aureus (MRSA) was found. Candida species were the commonest pathogens causing urinary tract infection (UTI). Mechanical ventilation, invasive device utilization, neonatal age, neutropenia, ICU residence and hospital stay for > 7 days were the risk factors significantly associated with HAI in our hospital. We found also, multiple antibiotic therapy usage, beta lactam usage, and ICU residence to be significant risk factors for infection by multidrug resistant pathogens. Conclusion: HAI is a significant problem. We recommend further nationwide multicenter studies to identify the commonest infectious pathogens, antibiotic resistance pattern, and to identify risk factors and pattern of antibiotic usage to overcome multidrug resistance