1 research outputs found
Affinity-Driven Immobilization of Proteins to Hematite Nanoparticles
Functional nanoparticles are valuable
materials for energy production,
bioelectronics, and diagnostic devices. The combination of biomolecules
with nanosized material produces a new hybrid material with properties
that can exceed the ones of the single components. Hematite is a widely
available material that has found application in various sectors such
as in sensing and solar energy production. We report a single-step
immobilization process based on affinity and achieved by genetically
engineering the protein of interest to carry a hematite-binding peptide.
Fabricated hematite nanoparticles were then investigated for the immobilization
of the two biomolecules C-phycocyanin (CPC) and laccase from <i>Bacillus pumilus</i> (LACC) under mild conditions. Genetic engineering
of biomolecules with a hematite-affinity peptide led to a higher extent
of protein immobilization and enhanced the catalytic activity of the
enzyme