Cryopreservation Studies in Aquaculture from Past to Present: Scientific Techniques and Quality Controls for Commercial Applications

In this section, cryopreservation of fish genetic resources, which is one of the important applications to ensure the sustainability of genetic resources of freshwater fish species, is discussed. At the same time, information is provided about the possible sources of contamination that may be encountered during cryopreservation applications. In this context, the results of sperm, egg, and embryo cryopreservation studies of fish and their success and failure in applications were evaluated in addition to the process from past to present. Information is given about the contamination that may develop depending on the applications in the process of cryopreservation and dissolving processes, as well as the studies carried out to eliminate extracellular disease agents. In the section, in addition to the evaluation of the results of scientific studies, commercial companies that commercially carry out gamete cryopreservation applications are also included. The contamination that may develop depending on the applications in the process of cryopreservation and thawing processes, as well as the studies carried out to eliminate extracellular disease agents are mentioned

An anatomical and histochemical examination of the pituitary gland of carp (Cyprinus carpio)

The present study was carried out for the purpose of studying the anatomical and the histochemical structure of the pituitary gland of the common carp, Cyprinus carpio. Anatomically, the shape of the gland has been observed to be round-oval, looking like an acorn. The pituitary gland consists of the adenohypophysis and neurohypophysis parts. Microscopically, the adenohypophysis consists of anterior (pars distalis) and posterior (pars intermedia) parts. The second lobe of the gland, called the neurohypophysis, is found in the core of the gland. Histologically, acidophilic prolactin cells stained red or orange and were dispersed in the rostral pars distalis (pro-adenohypophysis) of the adenohypophysis. Basophilic thyrotropin cells stained blue and were found in a small number in the same region. Adrenocorticotropin cells showed a chromophobic character; therefore, they did not get stained by the periodic acid-Schiff staining techniques. Gonadotropin cells were observed in the proximal pars distalis (meso-adenohypophysis) of the adenohypophysis. In the examined specimens, the faintly stained, elongated or pyramidal cells in the pars intermedia of the adenohypophysis were covering the neurohypophysis region. The neurohypophysial lobe was composed of unmyelinated nerve fibers and the pituicyte cells were located among the nerve fibers. Herring bodies were seen scattered in the neurohypophysis

Effects of environmental factors and food availability in Northern Aegean sea on the cultivation of Mediterranean Mussels (Mytilus galloprovincialis)

This study used two different collectors made of polypropylene (PP) rope and polyethylene (PE) fishing net to determine Mediterranean mussel's settlement, growth, condition index (CI), meat yield (MY) and shell thickness index (STI) during the period spent between June 2017 and November 2018. With this regard, chlorophyll a, water temperature, salinity, pH and particulate matter were defined through water samples that were collected on monthly basis triplicate. The mean chlorophyll a level was recorded as 0.32 +/- 0.31 mu g/L, while water temperature was 19.73 +/- 5.14 degrees C, salinity was 35 +/- 2.070 parts per thousand, pH was 8.12 +/- 0.04, and TPM was 14.91 +/- 10.48 mg/L. As a result of the cultivation period of 8 months spent following the first intense grip, the length of the mussels is measured as 31.79 +/- 6.20 mm. Based on the Pearson correlation analysis results, the most important environmental parameter affecting Mediterranean mussels growth in length on PP rope collectors is the temperature (p < .05). The STI and CI were determined to be related with environmental temperature parameters, while no determinations could be obtained concerning the correlation of MY with environmental parameters. Assessment of Mediterranean mussels' growth rate in PP rope collectors proved significant differences (p < .05) between March and November 2018. As no Mediterranean mussels are observed on PE fishing nets during the samplings performed throughout the study period, it is determined that PE fishing nets are not suitable collector types for Mediterranean mussel cultivation